Case report of oral melanoma in a king penguin (aptenodytes patagonicus)　found by cooperation of zoo and museum

Case report of oral melanoma in a king penguin (Aptenodytes patagonicus) found by cooperation between Kosei Inui1, Miyuki Hamaguchi1, Satomi Yonezawa1, Makiko Nishizawa1, Takeshi Wada1, Kazutoshi Takami2 1: Naniwa-Hone-Hone-Dan, Osaka Museum of Natural History 2: Osaka Municipal Tennoji Zoological Gardens AZEC 2013, Fukuoka

King penguin (Aptenodytes patagonicus) Sex and age: Adult male Body weight: 8.85Kg 1992 Nov. 28 Transferred to Tennoji Zoo (No previous Oral administration of antibiotics (trimethoprim) and application of anti- inflammatory drug (bromelain) was started as the continuous medical treatments for bumblefoot in the right plantar. Emaciation, inappetence, and dyspnea were observed. Antifungal drug was administered. Death. Brief necropsy was performed. Carcass was donated to Osaka Museum of Natural History (OMNH) to prepare flat skin During skinning, a black colored cauliflower- Reisfeld, L. et al. (2013) like mass was observed at the upper palate. Pesq. Vet. Bras. vol.33 AZEC 2013, Fukuoka

cauliflower-like mass AZEC 2013, Fukuoka

Histopathology: This mass was consisted of round to spindle tumor cel s containing with various amount of melanin pigment. AZEC 2013, Fukuoka

Human: Melanoma is a malignant neoplasm of melanocytes. Melanocytes produce the brown pigment called as melanin. Melanoma predominantly occur in the skin, but are also found in any part of the body that contains melanocytes. If skin melanoma is found early, and if it is completely removed surgically, then the chanc of cure is high. Veterinary field: Melanomas are relatively common in dog, horse, and certain breeds of swine, but less frequent in cattle and goats, and quite rare in cats and sheep. Melanomas are extremely rare in avian species. There are previous reports in fowls (1919, 1928, 1929 and 1932) emerald duck (1978), budgerigar (1991), macaroni penguin, red-tailed hawk (1996), and Humboldt penguin (1998 and 2003). This is the first case report of melanoma in king penguin as AZEC 2013, Fukuoka In the necropsy record in Tennoji Zoo, there is no description of specific changes including this tumor. Therefore, metastasis is unclear. Since the tumor located in the deep throat, it could not be find during the clinical phase and also the brief necropsy especial y focused on the body cavity. Osaka Municipal Tennoji Zoological Gardens Melanoma was found out during the skinning at the museum. Naniwa Hone-Hone Dan: Osaka Museum of Natural History A voluntary circle to prepare mammalian and bird specimens based in OMNH. Naniwa=Ancient Name of Osaka Hone=Bone Dan=Group AZEC 2013, Fukuoka Naniwa Hone-Hone Dan Activity:  Once or twice for a month (holidays)  In the practical room  No time-rule but no salary  Registered through qualification examination AZEC 2013, Fukuoka Bone Summit 2011 (11,100 participants) Osaka Bird Festival 2010 Bone Summit 2009 (8300 participants) Natural History Festival 2008 Osaka Bird Festival 2007 Exhibition of "Smal Zoo of Bones" at INAX Galleries" (2005 – 2006) Natural History Festival 2004 Receipt the specimens from Takarazuka Family Land Zoo AZEC 2013, Fukuoka Office worker, students, teachers, artists, and so on AZEC 2013, Fukuoka Where do carcasses come from? AZEC 2013, Fukuoka SOP (Standard Operating Procedures) - Mammalian - Measurement
Cleaning
Skinning
Necropsy
Illustration：
Makiko Nishizawa
AZEC 2013, Fukuoka SOP (Standard Operating Procedures) - Avian - Illustration：
Atsushi Satake
AZEC 2013, Fukuoka AZEC 2013, Fukuoka Number of specimens in OMNH Accept the specimens from Takarazuka Family Land Zoo AZEC 2013, Fukuoka Favorable voice from members：  To touch rare and beautiful animals  To know structure and function of animal body  To satisfy intellectual curiosity  Joy of discovery  To become much interested in biology  To increase knowledge  Satisfaction by accomplishment  To make friends who share sense of values regardless of age and gender  Improvement of technique  To became to love animals more  Homework of summer vacation AZEC 2013, Fukuoka Unfavorable voice from members：  Bad smell  Cannot confide to common people.  Needs patience  Far to commute  Zoonosis  To use protectors  Sterilization  Hygiene education Our perspective：  To tackle with more various species  To build up whole skeleton of large animal  To notify the value of carcasses of wild animals  To consider relationship of human and wildlife  Collaboration with other groups and other fields (ex. artists)  More efficient work by division of labor  Upbringing of the professional as next generation AZEC 2013, Fukuoka Voluntary citizen interested in animal science Utilization of specimens on 50,000 yen/specimen X 200/year = save 10,000,000 yen/year AZEC 2013, Fukuoka Kosei Inui, Yuka Tominaga, Yumi Honda, Makino Tachibana, Satomi Yonezawa, Makiko Nishizawa, Takeshi Wada, Hiroyuki ASZWM 2009 in Korea Case report of hypertrophic pulmonary osteoarthropathy observed in a Japanese raccoon dog (Nyctereutes procyonoides). El-Dakhly K, Inui K, Uranno N, El-Nahass el-S, Kakogawa M, Hirata A, Kimura J, Sakai H, Yanai T. J Zoo Wildl Med 2012 Dec; Occurrence of the subcutaneous mite, Hypodectes propus (Acarina: Hypoderatidae), in the grey heron, Ardea cinerea (Aves: Ciconi formes) a new host record in Japan. Avian subcutaneous mite AZEC 2013, Fukuoka Scabies in Japanese raccoon dog Relationship of human and wildlife Let's paint like human bones Chicken wing workshop AZEC 2013, Fukuoka Tohoku support: Delivery workshop (Osaka Natural History Center ) Tohno City (2011 Sep) Yamada-cho (2011 Sep and 2013 Nov) Ootsuchi-cho (2012 Dec) Oofunato City (2011 Sep, 2012 Dec and 2013 Sep) Rikuzen-takada City (2012 Dec and 2013 Nov ) Ichinoseki City (2013 Apr) Minami-sanriku-cho (2012 Oct, 2013 Jul and 2014 Feb) Sendai City (2014 Feb) Iwaki City (2012 June) AZEC 2013, Fukuoka Return to Osaka, we performed the workshop for children and parents in Osaka with same program:  Introduce of natural history of Sanriku area in Tohoku  Explain the significance of delivery workshop "We live apart each other but we are connected through this workshop."  Reconfirm the significance delivery workshop  Maintain motivation and improve the skil "Let's make use of new discovery in the next expedition." We were nominated for the best 6 candidates of the Civil Society Organization Award! AZEC 2013, Fukuoka  This is the first case of melanoma in king penguin found by cooperation between zoo and museum.  Naniwa Hone-Hone Dan consists of voluntary citizens who are interested in animal body.  Carcasses from zoo and aquarium are the most preferable specimen for members.  Activity of Naniwa Hone-Hone Dan can produce a complementary effect for zoo, museum and voluntary  Specimens, technique and intel igence are utilized for not only academic research but also education.  Our framework for cooperation between zoo/aquarium/ museum and citizen shows a good and rare example in the AZEC 2013, Fukuoka Thank you for your attention. Tissue preparation: Ms. Naoko Hongo (WDB, Co. Ltd.) Diagnostic advisory: Prof. Tokuma Yanai (Gihu Univ.) Mr. Atsushi Satake (Naniwa Hone- hone Dan) AZEC 2013, Fukuoka

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