Checkpoint kinase 1-mediated phosphorylation of cdc25c and bad proteins are involved in antitumor effects of loratadine-induced g2/m phase cell-cycle arrest and apoptosis

MOLECULAR CARCINOGENESIS 45:461–478 (2006) Checkpoint Kinase 1-Mediated Phosphorylationof Cdc25C and Bad Proteins Are Involved inAntitumor Effects of Loratadine-Induced G2/MPhase Cell-Cycle Arrest and Apoptosis Jinn-Shiun Chen,1 Shyr-Yi Lin,2 Wei-Ling Tso,3 Geng-Chang Yeh,4 Wen-Sen Lee,5 How Tseng,6Li-Ching Chen,7 and Yuan-Soon Ho3* 1Division of Colon and Rectal Surgery, Department of Surgery, Chang Gung Memorial Hospital, Linkou, Taiwan2Department of Internal Medicine, School of Medicine, Taipei Medical University, Taipei, Taiwan3Graduate Institute of Biomedical Technology, Taipei Medical University, Taipei, Taiwan4Graduate Institute of Medical Sciences, and Department of Pediatrics, School of Medicine, Taipei Medical University,Taipei, Taiwan5Graduate Institute of Medical Sciences, and Department of Physiology, School of Medicine, Taipei Medical University,Taipei, Taiwan6Graduate Institute of Medical Sciences, School of Medicine, Taipei Medical University, Taipei, Taiwan7Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan In this study, we first demonstrated that loratadine (LOR), a promising world widely used oral anti-histamine, effectively inhibits growth of tumors derived from human colon cancer cells (COLO 205) in an in vivo setting. In vitrostudy demonstrated that the anti-tumor effects of LOR in COLO 205 cells were mediated by causing G2/M phase cellgrowth cycle arrest and caspase 9-mediated apoptosis. Cell-cycle arrest induced by LOR (75 mM, 24 h) was associatedwith a significant decrease in protein levels of cyclin B1, cell division cycle (Cdc) 25B, and Cdc25C, leading toaccumulation of Tyr-15-phosphorylated Cdc2 (inactive form). Interestingly, LOR (75 mM, for 4 h) treatment alsoresulted in a rapid and sustained phosphorylation of Cdc25C at Ser-216, leading to its translocation from the nucleusto the cytoplasm because of increased binding with 14-3-3. We further demonstrated that the LOR-induced Cdc25C(Ser-216) phosphorylation was blocked in the presence of checkpoint kinase 1 (Chk1) specific inhibitor (SB-218078).
The cells treated with LOR in the presence of Chk1 specific inhibitor (SB-218078) were then released from G2/M arrestinto apoptosis. These results implied that Chk1-mediated phosphorylation of Cdc25C plays a major role in response toLOR-mediated G2/M arrest. Although the Chk1-mediated cell growth arrest in response to DNA damage is welldocumented, our results presented in this study was the first report to describe the Chk1-mediated G2/M cell-cyclearrest by the histamine H1 antagonist, LOR. ß 2006 Wiley-Liss, Inc.
Key words: loratadine; G2/M arrest; apoptosis; anti-tumor; Chk1 Cdc25C from ctyoplasm into the nucleus, and The cell cycle is controlled by the periodic checkpoint kinase 1 (Chk1), which phosphorylate regulation of the highly conserved cyclin dependent Cdc25C at Ser-216 and creates a consensus-binding kinases (CDKs) [1]. In eukaryotes, the G site for 14-3-3 [8]. The binding of 14-3-3 requires the point is controlled by the cell-division cycle (Cdc)2/Cyclin B complex, whose activity is required forentry into mitosis [2]. Previous studies indicate thatCdc2 (Tyr-15) phosphorylation is maintained during Abbreviations: Cdc 25B, cell-division cycle 25B; Chk1, checkpoint cancer therapeutic drug-induced G kinase 1; LOR, loratadine; ATR, ataxia telangiectasia-mutated and Rad 3-related; FCS, Fetal calf serum; DBH, debromohymenialdisine; mammalian cells [3–5]. Phosphorylation of Cdc2 DMSO, dimethylsulfoxide; PBS, phosphate-buffered saline; P.I., (Tyr-15) is accomplished by two major regulators propidium iodide; FACS, fluorescence activated cell sorting; pNA, including Wee1, which phosphorylate Cdc2 at Tyr- p-nitroaniline; CCCP, carbonyl cyanide m-chlorophenylhydrazone;Val, valinomycin; I.P., intraperitoneal; TF, terfenadine; ATM, ataxia 15 and Myt1, which phosphorylate Cdc2 at Thr-14 and, to a lesser extent, Tyr-15 [6]. The Cdc2 (Tyr-15) *Correspondence to: Graduate Institute of Biomedical Technol- was activated by Cdc25C, a dual specific phospha- ogy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110,Taiwan.
tase, whose activity is essential for entry into mitosis Received 20 June 2005; Revised 22 September 2005; Accepted [7]. In addition, two additional regulators include 14- 3-3, which binds Cdc25C and inhibits the import of DOI 10.1002/mc.20165 ß 2006 WILEY-LISS, INC.
phosphorylation of Cdc25C at Ser-216, and mutat- calf serum (FCS), penicillin/streptomycin solution, ing this residue to Ala abolishes the interaction [9].
and fungizone were purchased from Gibco-Life This site is present in the potential recognition motif Technologies (Paisley, UK). The chemical inhibitors for 14-3-3 and is phosphorylated in vitro by check- were obtained from various sources as indicated: The point kinases, such as Chk1 and Chk2 [10,11]. These Chk1 inhibitors SB-218078 [21], and the Chk1/Chk2 results leads to the nuclear export of Cdc25C and inhibitor debromohymenialdisine (DBH) [22,23] its subsequent cytoplasmic sequestration by 14-3-3 were from Calbiochem (San Diego, CA).
protein, which prevents the activation of the down-stream target of Cdc25C, the cyclin B/Cdc2 kinase that is responsible for G2/M transition. Thus, the The following monoclonal antibodies were obtain- association of 14-3-3 with target proteins could ed from various sources as indicated: anti-caspase-8, modulate cell-cycle progression through different anti-cytochrome C, anti-cyclin B1, anti-Chk1 (sc- mechanisms such as subcellular localization and 8408 or sc-7898), anti-Chk2 (sc-5278), anti-Cdc25B, enzyme activity, depending on cellular signaling.
anti-GAPDH, anti-AIF, and anti-14-3-3 antibodies It has been well demonstrated that Chk1 inhibi- (Santa Cruz Biotechnology, Santa Cruz, CA), anti- tion potentiate the cytotoxicity of DNA-damaging phosphor BAD (Ser-155) (Cell Signaling Technolo- drugs through abrogation of the cell-cycle check- gy, Beverly, MA), anti-PCNA (Dako Corporation, point [12–14]. However, several agents that including Denmark), anti-caspase 9, anti-caspase 3 antibodies anti-mitotic agent (paclitaxel) [15], topoisomerase II (Stressgen Biotechnologies, Victoria, BC, Canada), inhibitor (etoposide, camptothecin, and doxorubi- anti-cytochrome C oxidase, (Research Diagnostics, cin) [12,16], and microtubule-targeting agents Flanders, NJ), and anti-b-actin (Sigma Aldrich Che- (nocodazole) [17] which were not targeted on DNA mie GmbH). The rabbit polyclonal antibodies damage also revealed that G2/M arrest is largely against phospho-Chk1 (Ser-345), phospho-Chk2 dependent on Chk1-mediated signaling pathway. In (Thr-68), phospho-Cdc2 (Tyr-15), Cdc2, phospho- this study, the Chk1 was demonstrated to play some Cdc25C (Ser-216), and Cdc25C (C-20) were pur- important role in the loratadine (LOR)-induced chased from Cell Signaling Technology.
mitotic checkpoint. Our study also implied that theclinical efficacy of anti-tumorigenesis was expected Cells and Culture Conditions to be enhanced by Chk1 inhibitor, as evidenced byreleased of the G The COLO 205 cell line was isolated from human 2/M phase arrest cells into apoptosis.
In the present study, we demonstrate that LOR colon adenocarcinoma (American Type Culture treatment causes a G Collection CCL-222). The cell line FCH, a homo- 2/M phase cell-cycle arrest in COLO 205 cells which is associated with a marked zygous familial hypercholesterolemia cell (CRL 1831; decrease in the expression of key G American Type Culture Collection), was derived proteins, including cyclin B1, Cdc25B, and Cdc25C.
from primary cultures of normal CRLs [24]. The cells In addition, we provide evidence to indicate that were grown in RPMI 1640 supplemented with 10% cell-cycle arrest in LOR-treated COLO 205 cells is FCS, penicillin (100 U/ml), streptomycin (100 mg/ caused by ataxia-telangiectasia-mutated and Rad3- ml), and 0.3 mg/ml of glutamine for COLO 205; related (ATR) /Chk1-mediated phosphorylation of grown in DMEM/Ham's Nutrient Mixture F-12, 1:1 Cdc25C at Ser-216. Phosphorylation of Cdc25C in with 2.5 mM L-glutamine, 1.2 g/L sodium bicarbo- LOR-treated cells leads to its sequestration in the nate, 15 mM HEPES and 0.5 mM sodium pyruvate cytosol through increased binding with 14-3-3.
supplemented with 10% FCS, 10 ng/ml of cholera Recent studies demonstrated that ATR/Chk1 is toxin, 0.005 mg/ml of insulin, 0.005 mg/ml of postulated in response to DNA damage by ionizing transferrin, 100 ng/ml of hydrocortisone, and 10 mM radiation, UV light, or interference with DNA HEPES for CRL 1831 in a humidified incubator (378C, replication [18–20]. In this study, our results provide 5% CO2). LOR (Sigma Chemical Co., St. Louis, MO) further evidence showing that Chk1 signaling was added at the indicated doses in 0.05% dimethyl- regulatory proteins may be predominantly affect sulfoxide (DMSO). For control treated cells, the same the LOR-mediated G volume of DMSO was added in a final concentration cell-cycle checkpoint of 0.05% (v/v) without LOR.
MATERIALS AND METHODS Determination of Cell Viability COLO 205 and CRL 1831 cells were treated with Chemicals and Reagents LOR (10–75 mM). Cell viability was determined at the Protease inhibitors (phenylmethyl sulfonyl fluor- indicated times based on 3-(4,5-dimethylthiazol-2- ide (PMSF), pepstatin A, leupeptin, and aprotinin) were acquired from Sigma Chemical Company assay. Briefly, cells were seeded in a 96-well plate at (Sigma Aldrich Chemie GmbH, Steinheim, Germany).
a density of 1  104 cells/well and allowed to adhere Dulbecco's modified Eagle's medium (DMEM), Fetal overnight. After removing the medium, 200 ml of ANTITUMOR EFFECTS OF LOR IN HUMAN COLON CANCER CELLS GROWTH fresh medium per well, containing 10 mmol/L HEPES for translocation of cytochrome C from the mito- (pH 7.4) was then added. Then, 50 ml of MTT was chondria membrane into cytosol. Lysis of cells for added to the wells and the plate was incubated for mitochondrial protein extraction was performed in 2–4 h at 378C in the dark. The medium was removed isotonic buffer (200 mM mannitol, 70 mM sucrose, 1 and 200 ml DMSO and 25 ml Sorensens's glycine buffer mM EGTA, 10 mM HEPES, pH 6.9) by dounce was added to the wells. Absorbance was measured homogenization. Unbroken cells, nuclei, and heavy using an ELISA plate reader at 570 nm.
membranes were pelleted at 1000g for 5 min anddiscarded. The mitochondrial enriched fraction was Cell synchronization, drug treatment, and collected by pelleting at 12000g for 20 min. The flowcytometric analysis pellet was then washed briefly in alkaline wash buffer At 24 h after plating of cells, cells were washed (0.1 M Na2CO3, pH 11.5) to separate peripherally twice with phosphate-buffered saline (PBS) and then associated (alkali sensitive) mitochondrial proteins incubated with medium containing 0.04% FCS for from membrane integrated (alkali resistant) mito- additional 24 h. Under such conditions, cells were chondrial proteins and centrifuged. The pellet (con- arrested in G0/G1 as determined by using flowcyto- taining the membrane integrated proteins) was metric analysis [25]. After serum starvation, the low- resuspensed in RIPA lysis buffer (1 PBS, 1% Nonidet serum (0.04% FCS) medium was removed and the P-40, 0.5% sodium deoxycholate, 0.1% sodium cells were then challenged by addition of medium dodecyl sulphate) with protease inhibitor cocktail containing 10% FCS. LOR solutions were prepared in (Calbiochem) and used for immunoblotting. For a final concentration of 0.05% (v/v) DMSO. The cell- cytochrome C oxidase protein detection (cyto- cycle stages in the LOR and DMSO-treated groups chrome C oxidase, subunit IV, detection was were measured by flowcytometric analysis. Cells employed as a control to demonstrate that mito- were harvested and stained with propidium iodide chondrial protein fractionation was successfully (P.I.) (50 mg/ml) (Sigma Chemical Co.), and DNA isolated), equivalent samples (20 ml containing content was measured using a fluorescence activated approximately 50 mg protein) were separated by cell sorting (FACS) can laser flowcytometry analysis SDS–PAGE on 12% Tris glycine gels and transferred system (Becton–Dickinson, San Jose, CA); and 15000 to 0.2 mM polyvinylidene difluoride membranes events were analyzed for each sample.
(Invitrogen). Blots were probed with a mouse mono-clonal antiserum specific for cytochrome C (Santa Western Blotting Analysis Cruz, CA) or with a rabbit polyclonal antibody specific Western blotting analysis was performed as for cytochrome C oxidase followed by the appropriate described previously [26]. Briefly, cell lysates were secondary antibodies conjugated to horseradish per- prepared, electrotransferred, immunoblotted with oxidase (Santa Cruz) and then visualized by Super- antibodies, and then visualized by incubating with Signal chemiluminescence kit as described in the the colorigenic substrates (nitroblue tetrazolium, manufacturer's protocol (Pierce Biotechnology, Rock- NBT and 5-bromo-4-chloro-3-indolyl phosphate ford, IL) and visualized by autoradiography.
(BCIP)) (Sigma Chemical Co.). The expression of Preparation of Nuclear and Cytoplasmic Fractions either GAPDH, b-actin, or cytochrome C oxidase wasused as control for equal protein loading.
Nuclear and cytoplasmic fractions from control (DMSO-treated) and LOR-treated COLO 205 cellswere prepared as described previously [5]. Briefly, cells were harvested by scraping and rinsed twice in Equal amounts of protein were immunoprecipi- ice-cold PBS. The cells were then swollen in ice-cold tated with saturating amounts of anti-14-3-3 anti- hypotonic lysis buffer (20 mM HEPES, pH 7.1, 5 mM body. Immunoprecipitates were washed five times KCl, 1 mM MgCl2, 10 mM N-ethylmaleimide, 0.5 mM with extraction buffer and once with PBS. The pellet phenylmethylsulfonyl fluoride, 5 mg/ml of pepstatin was then resuspended in sample buffer (50 mM Tris, A, 2 mg/ml of chymostatin, 5 mg/ml of leupeptin, pH 6.8; 100 mM dithiothreitol; 2% SDS; 0.1% 5 mg/ml of aprotinin, 5 mg/ml of anti-pain) for 10 min.
bromophenol blue; 10% glycerol) and incubated The cells were lysed by 20 strokes in a Dounce for 10 min at 908C before electrophoresis to release homogenizer, and the nuclei were cleared by centri- the proteins from the beads. The 14-3-3-immuno- fugation (400g, 10 min). After this step, the super- precipitated phosphor Cdc25C and phosphor BAD natant (cytosolic fraction) was concentrated and proteins were then measured by Western blot stored at 808C. The nuclear extract was prepared analysis using specific antibodies.
using the same lysis buffer and stored at 808C priorto Western blot analysis for Cdc25C. The blot was Isolation of Mitochondria and Cytosolic stripped and reprobed with b-actin or PCNA antibody Fractions of Cell Lysates [27] to ensure equal protein loading as well as to rule out The COLO 205 cells were incubated with LOR (25 cross contamination of cytoplasmic and nuclear mM) for the indicated time points and then assayed Chk1 Kinase Activity Assay the lysis buffer and once with kinase buffer contain- Approximately 106 cells were plated at a conflu- ing 50 mM Tris-HCl (pH 7.4), 10 mM MgCl2, and ence of 70% and exposed to LOR (75 mM, 4 h). Cells 1 mM dithiothreitol. The beads were incubated with were collected, and Chk1 kinase activity was mea- 50 ml of kinase reaction mixture containing 50 mM sured by a immunoprecipitation kinase assay as Tris-HCl (pH 7.4), 10 mM MgCl2, 1 mM dithiothrei- described previously [28,29]. Briefly, the Chk1 assay tol, 10 mM ATP, 5 mCi of [g-32p] ATP, and 0.5 mg/ml was carried out in 50 ml volume in the following of histone H1 for 15 min. The reaction was buffer: 20 mM Na-HEPES, pH 7.4, 50 mM KCl, 1 mM terminated by the addition of 20 ml of 4 Laemmlis sample buffer and boiling for 5 min. The 32p- 2, and 1 mM DTT. A synthetic peptide with a sequence flanking Ser-216 of human phosphorylated histone H1 was separated by 0.1% Cdc25C, KVSRSGLYRSPSMPENLNRK, was used as its SDS, 10% polyacrylamide gel, and determined by substrate [29]. Chk1 was used at 5 nM, the peptide autoradiography using Kodak X-Omat film.
was used at 20 mM, and [g-32P]ATP was used at 10 mM(final concentration). Reactions were incubated at Analysis of Apoptosis 378C for 20 min and stopped by adding 5 ml of acetic Apoptosis in the COLO 205 cells subjected to acid containing 10 mM ATP. Thirty-microliter various treatments was determined by using the samples were spotted on 2-cm phosphocellulose DNA fragmentation analysis [33]. Briefly, the LOR filter disks, which were washed four times, 5 min and DMSO-treated cells were seeded on 100-mm each with 0.5% phosphoric acid and counted by dishes. The DNA was extracted twice with equal liquid scintillation. The apparent Km for ATP in the volumes of phenol and once with chloroform- Chk1 assay was about 120 mM.
isoamyl alcohol (24:1 v:v), precipitated with 0.1volume of sodium acetate, pH 4.8, and 2.5 volumes Wee1 Kinase Activity Assay of ethanol at 208C overnight, and finally centri- The recombinant Cdc2 protein was purified as a fuged at 13000g for 1 h. Genomic DNA was glutathione S transferase fusion protein containing quantitated, and equal amounts of DNA sample in full-length human Cdc2 (Santa Cruz Biotechnology) each lane were electrophoresed in a 2% agarose gel.
and then used as a substrate for Wee1 kinase assay.
The DNA was visualized by ethidium bromide Glutathione S-transferase protein was used as a negative control substrate and was prepared accord-ing to the standard procedure suggested by the Caspase Activity Assay manufacturer (Pharmacia, Piscataway, NJ). Prepara- Caspase activity was measured by using caspases 3 tion of cell lysates, immunoblotting, immunopreci- (Promega, Madison, WI) and 9 (Chemicon, Teme- pitation were performed as described previously [25] cula, CA) colorimetric activity assay kits as previous except that cell lysis buffer for immunoprecipitation described [34,35]. Briefly, COLO 205 cells were lysed was 50 mM Tris-HCL, pH 7.5; 1% Triton X-100; by addition of cell lysis buffer and protein concen- 0.5 mM Na3VO4; 50 mM sodium fluoride; 5 mM tration was measured. Caspase activity was assayed at sodium pyrophosphate; 10 mM sodium 2-glycero- 378C in 100 ml of assay buffer containing 50 mg (for phosphate; 0.1 mM PMSF; 1 mg/ml of aprotinin; 1 mg/ caspase 3) or 30 mg (for caspase 9) of the indicated ml of pepstatin; 1 mg/ml of leupeptin; 1 mM micro- colorimetric peptide. Caspase activity was measured cystin; 1 mM DTT; 1 mM EDTA, and 1 mM EGTA.
by the release of p-nitroaniline (pNA) from the Kinase assays for Wee1 were performed as described labeled substrates Ac-DEVD-pNA and Ac-LEHD- previously [30,31]. The Wee1 kinase assay used 500 mg pNA for caspase 3 and 9, respectively, and the free of cell extract for immunoprecipitating kinase, and pNA was quantified at 405 nm.
2 mg of relative protein substrate. Kinase reaction wascarried out by incubation for 30 min at 308C and Mitochondrial Transmembrane Potential Assay terminated by addition of 30 ml of 6 Laemmli SDS To assess the mitochondrial transmembrane sample buffer [32]. Substrate phosphorylation was potential (DCm), COLO 205 cells (1  106) were analyzed by SDS-polyacrylamide gel electrophoresis seeded in a six-well plate washed twice with PBS and then loaded with the cationic lipophilic fluor-ochrome JC-1 (5 mg/ml) for 10 min at 378C. Cells were Cdc2 Kinase Activity Assay washed twice with PBS and submitted to FACS- The method for the assay of the Cdc2 kinase was analysis. The red fluorescence of JC-1 aggregates according to our previous report [26]. The protein corresponds to the mitochondrial membrane poten- content in each sample was determined as described tial whereas the green fluorescence of JC-1 mono- above and adjusted to 100 mg/lane. Lysate (in 0.5 ml mers is indicative for the mitochondrial mass. Active of extraction buffer) was immunoprecipitated with mitochondria with high DCm accumulate JC-1 5 mg monoclonal anti-cyclin B1 antibody, the aggregates, which are red, whereas, in the mitochon- immuno-complexes were washed three times with dria with low DCm, JC-1 stays in a monomeric, green ANTITUMOR EFFECTS OF LOR IN HUMAN COLON CANCER CELLS GROWTH form. This renders the red/green ratio, a sensitive indicator of the mitochondrial DCm changes. Inaddition, carbonyl cyanide m-chlorophenylhydra- To investigate the antiproliferative effects of LOR, zone (CCCP; Calbiochem) or valinomycin (Val, human colon cancer cells (COLO 205) and human Sigma) were dissolved in 100% acetone and diluted normal CRL 1831 were treated with different doses in complete medium; the acetone concentration in (10–50 mM) of LOR for 1–5 days and the cell growth the medium did not exceed 1%. Both of the CCCP numbers were then determined (Figure 1A). LOR at and Val were added at a final concentration of 200 mM lower dose (10 mM) suppressed COLO 205 cell as a positive control, and the fluorescence was proliferation while at higher dose (25 mM) induced assessed for each time point, a red/green fluorescence cell death (Figure 1A, left). Interestingly, such effect ratio was then calculated. The mean red fluorescence was less profound when normal human colon of drug-treated cells was measured at 0, 2, 4, 6, 8, 10, epithelial cells (CRL 1831) treated with LOR (Figure and 12 h after LOR treatment, and presented as a 1A, right). These results indicated that colon cancer ratio of the absorbance in 590/535 nm [36].
cells were more susceptible to LOR treatment thannormal human CRLs.
Immunofluorescence Staining And To further demonstrate whether the cytotoxic Confocal Microscopic Observation effect induced by LOR was due to apoptotic cell For microscopic observations of nuclear/cyto- death. The LOR-induced apoptotic effect was eval- plasmic and nuclear/mitochondria distribution of uated by flowcytometric and DNA fragmentation Cdc25C and AIF, respectively, COLO 205 cells were analysis. As shown in the Figure 1B, both of the sub- incubated in 0.05% DMSO (control) or LOR for the G1 and G2/M phase populations were observed in indicated time points. COLO 205 cells were immu- COLO 205 cells exposed to 20–50 mM of LOR.
nostained with monoclonal antibodies specific to Interestingly, G2/M arrest instead of DNA fragmen- phosphor Cdc25C (Ser-216) and AIF (Santa Cruz, CA) tation was observed in COLO 205 cells, which for 2 h. After washing with PBS/Tween 20 for three exposed to higher dose (75 mM) LOR. Our previous times, monoclonal antibodies were visualized with report have demonstrated that apoptosis and G0/G1 goat-anti-mouse IgG antibody labeled with FITC cell-cycle arrest was induced by terfenadine (TF), an (green) for 1 h. Propidium iodide at concentration of histamine H1 receptor antagonist with a mechan- 100 ng/ml for 5 min was used for nuclear staining.
isms similar to LOR [37,38]. In order to demonstrate Stained cells were imaged by confocal scanning whether the LOR- or TF-induced apoptosis was microscopy (Olympus, Tokyo, Japan) using excita- mediated through the blockage of the specific tion/emission wavelengths of 458/488 nm and 543/ histamine H1 receptor signaling pathway, four 633 nm for FITC and P.I., respectively.
additional histamine H1 blockers including cetiri-zine, ebastine, epinastine, and fexofenadine [39] Treatment of COLO 205-Derived Xenografts In Vivo were added to COLO 205 cells (75 mM, 24 h) thendetected for DNA laddering effect. Our data demon- COLO 205 cells were grown in RPMI 1640 supple- strated that DNA fragmentation was observed only mented with 10% FCS as described in our previous in the LOR- and TF-treated group (Figure 1D). Such studies [25–27]. Cells were harvested through two results suggested that the histamine H1 receptor was consecutive trypsinizations, centrifuged at 300g for not the major apoptosis-signaling sensor in response 5 min, washed twice, and resuspended in sterile PBS.
to LOR in the COLO 205 cells.
Cells (5  106) in 0.2 ml were injected subcuta- To address the cell-cycle effects of LOR, the COLO neously between the scapulae of each nude mouse 205 cells were synchronized at the G0/G1 phase by (purchased from National Science Council Animal 0.04% serum starvation for 24 h [25,26]. After serum Center, Taipei, Taiwan). After transplantation, starvation, the complete medium containing 10% tumor size was measured using calipers and the FCS was then replaced. The cells treated either with tumor volume was estimated according to the mock-treated (DMSO) or LOR (75 mM) were then formula tumor volume (mm3) ¼ L  W2/2, where L measured for cell cycle by flowcytometric analysis.
is the length and W is the width [26]. Once tumors As shown in Figure 2A (right), G2/M cell-cycle arrest reached a mean size of 200 mm3, animals received was observed initially at 9 h and reached the intraperitoneal (I.P.) injections of either 25 ml DMSO maximal level of more than 40% at 24 h after LOR or 25 mg/kg LOR three times per wk for 6 wk.
It is well established that onset of mitosis is triggered by activation of the Cyclin B1/Cdc2 kinase, Statistical analysis was carried out using analysis of which is absolutely required for transition of G2 cells variance (ANOVA)—one way analysis of variance into M phase [19,40]. During the G2 phase, Cyclin with Student—Newman correction, and the Stu- B1/Cdc2 is inactivated by phosphorylation at the dent's t-test. Significance was assumed for values of Thr-14 and Tyr-15 residues, which are dephosphory- P < 0.05.
lated by Cdc25C phosphatase before going into

Figure 1. Cell growth inhibitory effects of LOR on human colon using established CellFIT DNA analysis software. Three samples were cancer and normal epithelial cells. (A) Human colon cancer (COLO analyzed in each group, and values were presented as mean  SE. (C) 205) cells and human normal CRL 1831 were treated with various COLO 205 cells were treated with LOR (10–75 mM) or DMSO concentrations of LOR (10–50 mM) for 5 days. Media with or without (0.05%), and DNA fragmentation assay performed 24 h later. M, LOR were changed everyday until cell counting. Three samples were molecular weight marker. (D) COLO 205 cells were cultured in media analyzed in each group and the results were presented as supplemented with 10% FCS in the presence of histamine H1 means  SE. (B) Dose-dependent response of LOR on cell-cycle receptor antagonists namely LOR, TF, cetirizine, ebastine, epinastine, regulation. COLO 205 cells were cultured in media supplemented and fexofenadine (75 mM each treated for 24 h). DNA fragmentation with 10% FCS and LOR (10–75 mM) for 24 h. Percentage of cells in analysis was then performed as described above.
sub-G1, G0/G1, S, and G2/M phases of the cell cycle were determined mitosis. In this study, our results indicated that cells [26,41] were selected as 0 h (representing the G0/G1 arrested at the G2/M phase by 24 h of LOR (10–75 mM) phase), 15 h (representing the S phase), 18 h treatment were regulated by inhibition of the (representing the G2/M phase), and 24 h (represent- cyclin B1/cdc2 protein levels and its kinase activity ing the 2nd G0/G1 phase). The cell lysates were then (Figure 2B). In order to examine the time-dependent analyzed for expression of cell-cycle regulatory effect of LOR on G2/M phase cell-cycle arrest, the proteins by immunoblotting using specific antibo- COLO 205 cells were synchronized at the G0/G1 dies (Figure 2C). To assess the Cdc2 kinase activity, phase by 0.04% serum starvation for 24 h [25,26].
the level of phosphorylated Cdc2 (Tyr-15) as well as After serum starvation, the complete medium con- the level and/or kinase activity of the Wee1 and taining 10% FCS was then replaced. The time points Cdc25C phosphatase, two key enzymes that regulate according to Figure 2A and our previous studies the Cdc2 kinase activity, were determined in the

ANTITUMOR EFFECTS OF LOR IN HUMAN COLON CANCER CELLS GROWTH Figure 2. Effects of LOR on cell-cycle regulations in human COLO regulatory proteins in COLO 205 cells. The cells were rendered 205 cells. (A) Time-dependent effects of LOR in human COLO 205 quiescent by incubation for 24 h in the cultured media containing cell-cycle analysis. FACS analysis of DNA content was conducted 0.04% FCS. After 24 h, cells were released from quiescence by after COLO-205 cells release from quiescence by incubation in incubation in culture media supplement with 10% FCS and 0.05% culture media supplemented with 10% FCS in the presence of DMSO with or without LOR (10–75 mM) for additional 24 h. The cells 0.05% DMSO or LOR (75 mM) at the indicated time points.
were harvested, lysed, and the levels of G2/M phase regulatory Percentage of cells in different phases of cell cycle was determined proteins and its kinase activities were determined as described in the using established CellFIT DNA analysis software. Three samples were Materials and Methods. Membrane was also probed with anti- analyzed in each group, and values represent the mean  SE. (B) GADPH antibody to correct for difference in protein loading.
Dose-dependent effects of LOR on the expression of G2/M phase LOR-treated COLO 205 cells. The results indicated The Chk1 is a serine-threonine kinase that is that phosphorylation of Cdc2 (Tyr-15) and its kinase critical for G2/M arrest in response to DNA damage.
activity was changed as early as 15 h after LOR Recently, it has been demonstrated that Chk1 (75 mM) treatment (Figure 2C). Consistent with the inactivate the pro apoptotic protein BAD by phos- effect on phosphorylation of Cdc2 (Tyr-15), LOR phorylating residues critical for BAD functions treatment also resulted in a marked induction of in vitro [46]. In this study, our results demonstrated Wee1 kinase activity in the same levels of total Wee1 that BAD (Ser-155) was phosphorylated as early as 1 h protein (Figure 2B and C). In addition, the LOR in COLO 205 cells by LOR treatment (Figure 3A).
treatment in COLO 205 cells also resulted in a Because phosphorylation of Cdc25C (Ser-216) [43] significant reduction in Cdc25C protein levels and BAD (Ser-155) [47] creates a binding site for 14-3- (Figure 2B and C). Thus, the induction of Wee1 3, we then examined the effect of LOR on the binding kinase at 15 h after LOR (75 mM) treatment in COLO of Cdc25C and BAD with 14-3-3 (Figure 3C). The 205 cells was associated with the decreased of the lysate proteins from control and LOR-treated cells Cdc25C phosphatase activity which eventually (10–75 mM for 4 h) were immunoprecipitated using increased the level of phosphorylated Cdc2 (Tyr-15) anti-14-3-3 antibody, and the immune complex was (Figure 2C). The net effect of the results is a G2/M analyzed for the presence of Cdc25C and BAD by arrest following LOR treatment.
immunoblotting. As can be seen in Figure 3C, LOR Chk1 and Chk2 are intermediaries of DNA damage treatment resulted in increased binding of Cdc25C checkpoints and activated by phosphorylation on and BAD with 14-3-3 at the 4 h time point. These Ser-345/Ser-317 and Thr-68, respectively [20,42].
results suggested that LOR treatment might lead to The Chk1 and Chk2 kinases have been reported to translocation of Cdc25C from the nucleus to the inhibit Cdc25C kinase activity by phosphorylation at cytoplasm because of increased binding with 14-3-3.
the Ser-216 residue [43–45]. We therefore examined We examined this possibility by immunohistochem- whether LOR treatment affects the phosphorylation istry, and the data were shown in Figure 3D. Cells of Chk1 or Chk2. Representative immunoblots for were treated with DMSO (control) or 75 mM LOR for 4 phospho-Chk1 showed increased Ser-345 phosphor- h, and then stained with anti-pCdc25C (Ser-216) ylation of Chk1 at 15 h after LOR (75 mM) treatment, antibody (FITC, green) or nucleic acid binding dye whereas the level of total Chk1 proteins were not P.I., red. In LOR (75 mM, 4 h)-treated cells, pCdc25C affected (Figure 2C). The total and its phosphory- (Ser-216) was localized in the cytoplasm (green lated form of the Chk2 (Thr-68) proteins did not staining surrounding P.I.-stained nuclei) as well as changed in the COLO 205 cells even at 24 h after LOR in the nucleus (red staining in nucleus). In contrast, (75 mM) treatment (Figure 2B and C).
the nuclei of the control cells were stained with To investigate the early stage responses of the pCdc25C (Ser-216) (green), indicating translocation COLO 205 cells for LOR treatment, the total and its of pCdc25C (Ser-216) from the nucleus to the phosphorylated form of the Chk1 (Ser-345), Chk2 cytoplasm (Figure 3D). Cytoplasmic accumulation (Thr-68), and Bad (Ser-155) were then determined. As of pCdc25C (Ser-216) upon treatment with LOR was shown in the Figure 3A, our study demonstrated that confirmed by biochemical fractionation of cytoplas- Chk1 (Ser-345) but not Chk2 (Thr-68) was phos- mic, and nuclear fractions from control (DMSO- phorylated in the COLO 205 cells as early as 1 h when treated) and LOR treated (75 mM for 4 h) cells exposed to higher dose LOR (75 mM). The kinase followed by immunoblotting using anti-pCdc25C activity of Chk1 was then determined in the lysates (Ser-216) antibody, and the results were shown in prepared from DMSO- and LOR-treated cells (75 mM Figure 3E. The total protein level of Cdc25C was also for 4 h) using GST-Cdc25C as a substrate. As can be determined in different fractions of the LOR- and seen in the Figure 3B, the Chk1 kinase activity was control-treated cells, which represented as a protein significantly higher in LOR-treated cells than in loading control. A 4 h time point was selected to control cells.
minimize influence of LOR induced decline in Figure 3. Effect of LOR on binding of Cdc25C and BAD with 14-3- (10–75 mM for 4 h) were used for immunoprecipitation with anti-14- 3 and on nuclear/cytoplasmic distribution of Cdc25C. (A) Immuno- 3-3 antibody followed by Western blotting (WB) for phosphor blotting assay for effect of LOR on protein level, and phosphorylation Cdc25C (Ser-216) and phosphor BAD (Ser-155). (D) Confocal of Chk1 (Ser-345) and BAD (Ser-155). COLO 205 cells were cultured microscopic analysis for nuclear/cytoplasmic distribution of Cdc25C in the presence of 75 mM LOR for the indicated time periods. The (Ser 216) in control (DMSO-treated) and LOR-treated cells. Cells were blots were stripped and reprobed with anti-GAPDH antibody to treated with DMSO (control) or 75 mM LOR for 1 or 4 h and then ensure equal protein loading. (B) Effect of LOR on Chk1 kinase stained with anti-Cdc25C (Ser-216) antibody (green) or P.I. (red). (E) activity. COLO cells were treated with DMSO or 75 mM LOR for 4 h.
Immunoblotting assay for pCdc25C(Ser-216) and Cdc25C using Chk1 was immunoprecipitated from the lysates of control and LOR- nuclear and cytoplasmic fractions prepared from control (DMSO- treated cells, and the kinase activity was determined using treated) and LOR-treated cells (75 mM for 4 h). Blots were stripped synthesized Cdc25C peptide as a substrate described in Materials and reprobed with anti-b-actin and anti-PCNA antibodies to and Methods. The membrane was probed with anti-Chk1 antibody normalize for equal protein loading as well as to rule out cross to ensure equal protein loading. (C) Effect of LOR on binding of contamination of the nuclear and cytoplasmic fractions. [Color figure phosphor Cdc25C (Ser-216) and phosphor BAD (Ser-155) with 14-3- can be viewed in the online issue, which is available at www.
3. The protein lysates (200 mg) from control and LOR-treated cells

ANTITUMOR EFFECTS OF LOR IN HUMAN COLON CANCER CELLS GROWTH Cdc25C protein level shown in the Figure 2B. In containing 4N-DNA (G2/M phase cells) (Figure 4B, DMSO-treated control, the intensity of Cdc25C immunoreactive band was significantly higher in To further clarify the role of checkpoint kinases the lane corresponding to nuclear fraction than in activation on LOR-mediated G2/M arrest, two inhi- the cytoplasmic fraction (Figure 3E). Treatment of bitors were adapted in our experiment including cells with LOR (75 mM) resulted in a decrease in DBH [48] and caffeine [49–51], which inhibited nuclear Cdc25C signal intensity with a concomitant the Chk1/Chk2 and ataxia telangiectasia-mutated increase in cytoplasmic Cdc25C signal intensity (ATM)/ATR kinases, respectively. Interestingly, the (Figure 3E). The blot was stripped and reprobed with LOR-mediated G2/M arrest was completely attenu- anti-b-actin and anti-PCNA antibodies to determine ated by DBH (10 mM) (Figure 4C, gray bars), but not cross contamination, if any, of the nuclear and by caffeine (5 mM) (Figure 4C, open bars). Such cytoplasmic fractions and to ensure equal protein results implied that at least part of the LOR-induced loading (Figure 3E). These results confirmed that G2/M arrested cells utilize a caffeine-insensitive LOR treatment, indeed, promoted translocation of pathway in which checkpoint signaling by ATM or Cdc25C from the nucleus to the cytoplasm.
ATR was most likely not involved.
To determine the possible role of Chk1 kinase To examine the effect of Chk1 on LOR-mediated activation in the regulation of LOR-mediated G2/M apoptotic effects, COLO 205 cells were treated with arrest, we examined the effects of Chk1 kinase on LOR (25 and 75 mM) and then incubated for 24 h in LOR-induced G2/M arrest in COLO 205 cells using the medium containing 0.1–1 mM SB-218078 (Figure Chk1-specific inhibitor SB-218078 [21]. We first 4D, lanes 5 and 6) or, as a control, 0.05% DMSO demonstrated that SB-218078 (0.1 mM) inhibited (Figure 4D, lane 1). The presence of DNA fragmenta- the phosphorylation of Chk1(Ser-345) and its kinase tion was detected as described above. The dose of SB- activity in COLO 205 cells when treated with LOR 218078 used in this study (0.1–1 mM) was found to (Figure 4A). The inhibitory effects of SB-218078 on maximally prevent the LOR-induced G2/M arrest, LOR-induced Cdc25C, Cdc2, and BAD phosphoryla- which resulted in DNA laddering formation (Figure tion were demonstrated (Figure 4A). To determine 4D, lanes 5 and 6). As a control, a parallel set of COLO the effective dose of SB-218078 on LOR-mediated G2/ 205 cells was incubated in the presence of 1 mM SB- M arrest response, COLO 205 cells were treated with 218078, no significant DNA fragmentation was LOR and then cultured for 24 h in the presence of observed (Figure 4D, lane 2).
SB-218078 (0.1 and 1 mM). The resulting cells were Our results revealed that lower dose (10–50 mM) analyzed for DNA contents by FACS flowcytometric LOR could induced both the G2/M arrest and analysis as described in Materials and methods. The apoptosis in COLO 205 cells (Figure 1B and D). The results showed that the LOR-induced G2/M arrested mechanisms of LOR-mediated apoptosis need to be cells (Figure 4B, solid bar) was 3.9-fold higher than further investigated. Since the mitochondria signal- that in the control cells (Figure 4B, open bar).
ing regulatory proteins seems to be required for Furthermore, incubation of LOR-treated COLO different anti-cancer agents-induced apoptosis in 205 cells with SB-218078 resulted in a marked COLO 205 cells [35,52–54]. We first examined attenuation of LOR-induced G2/M arrest in a dose- whether cytochrome C release from mitochondria dependent manner (Figure 4B, solid bars). Incuba- into the cytosol and dissipation of the electrochemi- tion with 0.1 mM SB-218078 inhibited the LOR- cal gradient (DCm) was involved in the LOR- induced G2/M arrest by approximately 50%, while mediated apoptosis. This was monitored by staining incubation with 1 mM SB-218078 diminished the the COLO 205 cells with JC-1, a fluorescent dye, LOR-induced G2/M cell-cycle arrest by 80% (Figure 4B, which differentially stains mitochondria in accor- solid bars). No further inhibitory effect on LOR- dance to their DCm. Active mitochondria with high induced G2/M arrest was seen when higher doses of DCm accumulate JC-1 aggregates, which are red SB-218078 were used (data not shown). Incubation (Figure 5A, 0 h), whereas, in the mitochondria with of DMSO-treated COLO 205 cells with SB-218078 low DCm, JC-1 stays in a monomeric, green form had no detectable effect on the population of cells (Figure 5A, 12 h). This renders the red/green ratio, a Figure 4. The Chk1 regulatory effects of LOR-induced G2/M cell content, indicative of G2/M phase of the cell cycle, is shown as the cycle arrest. (A) COLO 205 cells were treated with LOR (75 mM) in the mean  SE from three independent experiments. (C) COLO 205 cells presence of SB-218078 (0.1 and 1 mM) for 24 h. The treated cells were treated with LOR (75 mM) followed by incubation for 24 h in were collected, lysed, and the level of phosphorylated Chk1 (Ser- medium containing 0.05% DMSO (solid bars), 5 mM caffeine (open 345), Cdc25C (Ser-216), Cdc2 (Tyr-15), and BAD (Ser-155) as well as bars), or 10 mM DBH (gray bars). Cell samples were analyzed for DNA the level of total Chk1, Cdc25C, Cdc2, and BAD in each sample was content by flowcytometry. The percentage of G2/M phase cells determined by immunoblotting analysis with relevant specific shown represents the average of three independent experiments. (D) antibodies. The Chk1 kinase activity was determined as described COLO 205 cells were incubated for 24 h in medium containing in Materials and Methods. (B) Cells were treated with DMSO (open 0.05% DMSO, SB-218078 alone, or combine treatment with LOR bars) or LOR (75 mM) (solid bars), and incubated for 24 h in the and SB218078. The DNA samples were isolated from cells and presence of Chk1 inhibitor (SB-218078). The resulting cells were analyzed for DNA fragmentation as described in the Material and harvested, stained with PI and analyzed for DNA contents by flowcytometry as described above. Percentage of cells with 4N-DNA


ANTITUMOR EFFECTS OF LOR IN HUMAN COLON CANCER CELLS GROWTH sensitive indicator of the mitochondrial DCm ptotic responses by which the initiator (caspase 9 changes, which does not depend on other factors and 8) and the effector (caspase 3) were determined such as mitochondrial size, shape, and density, by immunoblotting and caspase activity analysis which may influence single-component fluorescent (Figure 6A and B). The COLO 205 cells were treated signals. Analyzed in a real-time plate reader assay, with various concentrations of LOR (10–75 mM) for DCm stayed relatively stable in untreated COLO 205 24 h. Our data demonstrated that LOR at a lower dose cells, while it was rapidly (within 6 h) dissipated by (25–50 mM) activated the caspase 3 as evidenced 25 mM LOR treatment (Figure 5A, lower panel). The by a decreased in the protein level of procaspase 3 DCm was rapidly (within 2 h) dissipated by the and degradation of the poly-ADP-ribose polymerase uncoupler CCCP or by the Kþ ionophore Val and (PARP), the substrate for caspase 3 (Figure 6A). To served as a positive control (Figure 5A). As shown in further elucidate the apoptotic pathways involved in the Figure 5A and B, we found that LOR (25 mM) the activation of caspase 3, we examined the changes increased outer (cytochrome C and AIF release) and of the protein levels of caspases 8 and 9 in the LOR- inner (loss of DCm) mitochondria membrane per- treated COLO 205 cells. Treatment of COLO 205 cells meability. The release of cytochrome C and AIF were with LOR (25–50 mM) resulted in caspase 9 activation then observed as early as 6 h which kinetically evidenced by degradation of the procaspase 9 but paralleled a decreased of mitochondria membrane not by caspase 8 because substrate of the caspase 8 potential (Figure 5A and B). These observations (truncated form of Bid, t-Bid) was not degraded suggest that LOR-induced apoptosis may be initiated (Figure 6A). These observations suggest that LOR- with early alterations in mitochondrial membrane induced apoptosis is dependent on caspase 9 activa- tion. Activation of caspase 9 in the Apaf-1 apopto- As shown in the Figure 5B, the AIF was released some is predominantly triggered by the release of from mitochondria and translocated into nucleus as cytochrome C from mitochondria into the cyto- early as 6 h after LOR treatment in the COLO 205 plasm [56]. Accordingly, the cytosolic and mito- cells. To our knowledge, AIF was demonstrated to be chondrial fractions, prepared from cells treated with involved in initiate the nuclear apoptotic events LOR (25 mM) time-dependently were subjected to including nuclear membrane degradation, periph- Western blot analysis to assess the release of cyto- eral chromatin condensation, large-scale fragmenta- chrome C and demonstrated that the LOR induced a tion of DNA, and, ultimately, cytotoxicity [55]. We significant increase in cytosolic levels of cytochrome then confirmed the LOR-induced nuclear transloca- C as early as 6 h after LOR treatment (Figure 5B). To tion of the AIF by immunofluorescence staining further confirm the Western blot results, we perfor- observed by confocal microscopic analysis. In con- med caspase activity assays. As shown in Figure 6B, sistent to the result shown in Figure 5B, our data treatment of COLO 205 cells with 25 mM LOR indu- demonstrated that AIF translocation to nucleus was ced caspase 3 activity about 7.8-folds and 11.2-folds found in COLO 205 cells at 6 h after LOR (25–50 mM) at 9 and 12 h compared to control, respectively, treatment. As shown in the Figure 5C, the AIF while induced caspase 9 activity about 5.1-folds translocation to nucleus was not observed in COLO and 7.8-folds at 9 and 12 h compared to control, 205 cells after treated with higher dose of LOR respectively. We therefore hypothesized that LOR- (75 mM). Similar result was observed in the Figure 1B mediated apoptosis was through the mitochondria indicated that LOR-induced DNA fragmentation was signaling pathways in which the caspase 9 protein not observed in the COLO 205 cells when exposed to may be activated.
higher dose (75 mM) LOR. Such observations indicat- We next examined the therapeutic efficacy of LOR ing that higher dose LOR may trigger some mediators (25 mg/Kg) in vivo by treating athymic nude mice which induced G2/M cell growth arrest effects and bearing COLO 205 tumor xenografts. After establish- may contribute to inhibit of cellular apoptosis stress ment of palpable tumors (mean tumor volume, 200 mm3), animal received I.P. injections of LOR To investigate the molecular events of LOR- three times per wk, as well as DMSO plus peanut oil as induced apoptosis, we then investigated the apo- a vehicle control. After 6 wks, gross morphology of Figure 5. Evaluation on the role of the mitochondria signals AIF released form mitochondria into the cytoplasmic and nuclear involved in LOR induced apoptosis. (A) The top insets, mitochondria were then determined by Western blot analysis. The expression levels membrane depolarization in LOR-treated COLO 205 cells was of the cytochrome C oxidase (subunit IV), GAPDH, and PCNA measured by JC-1 staining. In the lower panel, COLO 205 cells were proteins were employed as a loading control to rule out cross treated with LOR (25 mM), Val (200 mM), or with CCCP (200 mM) for contamination of the mitochondria, cytoplasmic, and nuclear the indicated time points. After drug treatment, the COLO 205 cells fractions, respectively. (C) Confocal microscopic analysis for were stained with JC-1 (1 mg/ml) as described in Materials and nuclear/cytoplasmic distribution of AIF in control (DMSO-treated) Methods. Results were expressed as a change in the ratio between and LOR-treated cells. Cells were treated with DMSO (control) or LOR red JC-1 fluorescence (Em 590 nm) and green JC-1 fluorescence (Em (25–75 mM) for 4 h and then stained with anti-AIF antibody (green) 535 nm) over time. Each point represents the mean  SE from three or P.I. (red). [Color figure can be viewed in the online issue, which is independent experiments. (B) COLO 205 cells were treated with LOR available at] (25 mM) time dependently. The protein level of the cytochrome C and

significance of application for cancer chemothera-peutic purposes.
LOR, which is a promising world widely used oral- anti-histamine agent, has been used in the treatmentof allergic disease. In this study, we have shown thatLOR effectively inhibits proliferation of humancolon cancer (COLO 205) cells by causing G2/Mcell-cycle arrest and caspase 9-mediated apoptosis. Invivo study revealed that the growth of COLO 205xenografts in nude mice was retarded significantlyupon I.P. administration of LOR with a clinicaltherapeutic relevance dose (25 mg/kg). These resultsprompted us to examine further the mechanisms bywhich LOR inhibits proliferation of cancer cells.
Recent studies demonstrated that cells response to DNA damage agents initiated two distinct check-point kinases namely Chk1 and Chk2 [18,57].
Recently, several Chk1 and Chk2 checkpoint abro-gating agent (abrogator) have been developed asadjuvants and intended to improve the therapeuticindex of cancer chemotherapy [23,58]. The originalstudies were performed by using caffeine, a non-specific G2 abrogator which disrupt the G2 check-point to sensitize G1-defective cancer cells intoapoptosis [59,60]. In our study, the Chk1 specificinhibitor (SB-218078) were used as a G2 phaseabrogator and demonstrated that SB-218078 abro-gate the LOR-induced G2/M arrest and sensitized thecells from G2/M arrest into apoptosis (Figure 4D).
Additional studies also demonstrated that knock-down of Chk1, Wee1, and Myt1 by RNA interferenceabrogates either adriamycin- or paclitaxel-mediatedG2 checkpoint and induces apoptosis in humancancer cells [17,58,61]. Therefore, Chk1 downregu-lation can not only potentiate DNA-damagingagents, but also enhance the toxicity of anti-micro-tubule agents (such as paclitaxel) [12,17,58], which Figure 6. Dose-dependent response of caspase activity involved in significantly broadens its therapeutic applications.
LOR-mediated apoptosis in COLO 205 cells. (A) COLO 205 cells were In our study, the LOR-induced G2/M phase arrest treated with LOR (10–75 mM) for 24 h and the expression levelsof caspase-associated proteins were determined by Western blot was completely attenuated by SB218078 and DBH analysis. The expression level of the GAPDH protein was selected and but not by caffeine (Figure 4B). Caffeine has been as a loading control. (B) COLO 205 cells were treated with 25 mM shown to inhibit the activities of both ATM and ATR LOR for the indicated time points. The LOR-induced caspase activitiesin COLO 205 cells were measured as described in Materials and upstream checkpoint kinases and to override the Methods. Data was represented as mean  SE from three indepen- ATM- and ATR-dependent DNA damage checkpoints [49]. Recently, a cell-free experimental system fromXenopus eggs extracts was performed and described a the tumor volume in mice treated with LOR (Figure caffeine-insensitive pathway which plays some 7A, mice No. 5–8) was significant smaller in important role in the checkpoint response [62,63].
comparison with DMSO-treated controls (Figure Similar reports in human colon cancer cells also 7A, mice No. 1–4). A reduction in tumor volume demonstrated that ATR-mediated DNA-damage between mice given LOR versus those given vehicle checkpoint was not completely rescued by caffeine.
(DMSO) was detected (Figure 7B–E). In mice receiv- For example, increased phosphorylation of gH2AX ing these treatment regimens, no gross signs of was regulated by ATR in response to DNA breaks toxicity were observed (body weight, visible inspec- observed in human colon cancer cells [64]. The tion of general appearance, and microscopic exam- phosphorylated gH2AX was also induced in human ination of individual organs). Our results provide colon cancer (HCT 116) cells by DNA alkylating further evidences that such observations may have agent, hedamycin. In this study, caffeine did not

ANTITUMOR EFFECTS OF LOR IN HUMAN COLON CANCER CELLS GROWTH Figure 7. The growth of COLO 205 tumor xenografts in nude (shown as No. 5–8) for 6 wk. (B) Average tumor volume of DMSO- mice was retarded by LOR treatment. Athymic nude mice injected treated (circle) versus LOR-treated (square) nude mice, (C) body with COLO 205 cells into subcutaneous tissue of inter-scapular area.
weight, (D) tumor weight, and (E) tumor/body weight ratio were Once tumor volume reached approximately 200 mm3, the animal measured at the end of the experiment. Four samples were analyzed received treatment of 25 mg/kg LOR, or DMSO intraperitoneally in each group and values represent the mean  SE. Comparisons three times per wk for 6 wk. (A) Gross appearance of subcutaneous were subjected to Student's t test. *P < 0.05 versus control.
tumors after treatment with DMSO (shown as No. 1–4) or LOR substantially reduce induction of H2AX phosphor- not completely reverse the LOR-induced G2/M arrest ylation by hedamycin [65]. The H2AX phosphoryla- (Figure 4C). These results suggest that a caffeine- tion was also induced by silibinin, a naturally sensitive pathway responsible for maintenance of occurring flavonoid, which against ultraviolet B the LOR-induced G2/M-arrested cells is dependent (UVB)-induced skin tumorigenesis. However, the on both ATR and Chk1 checkpoint kinases. In such a anti-tumor effects of silibinin is possibly not through situation, the G2/M arrest induced by LOR can be ATM/ATR activation, as caffeine pretreatment had prevented by caffeine pretreatment. However, our no effect on silibinin plus UVB induced p53-Ser15 data indicated that at least part of LOR-induced G2/M arrested cells utilize a caffeine-insensitive pathway, As described above, caffeine is a well-known that is, that checkpoint signaling by ATM or ATR is inhibitor of both ATM and ATR pathways, it could most likely not involved. This result cannot exclude a possibility indicated that a distinct, caffeine- the residue of Ser-155 [46], which results in seques- insensitive ATM/ATR signaling pathway might also tering BAD from mitochondria into cytosol by be necessary to attenuate the effects of LOR- interacting with 14-3-3 [75]. The LOR-mediated mediated G2/M arrest and also plays a role in the Chk1 kinase activity was inhibited by SB-218078 which prevent the formation of p-BAD/14-3-3 com- In our and others previous studies [12,26,41], plex and promoted the G2/M phase arrested cells into the cyclin B1 protein was induced in the microtubule apoptosis in the COLO 205 cells (Figure 4A).
damaging agents-mediated G2/M arrested cells due In summery, the results of this study provide to block of the mitotic process. However, decreased evidence that LOR-induced G2/M arrest in COLO of cyclin B1 protein level was also observed in the 205 cells involves regulation of Cdc2 activity G2/M arrested cells through inhibition of the mRNA through three distinct mechanisms, which include synthesis and accelerate degradation of the cyclin B1 LOR-induced activation of Chk1 kinase and LOR- protein [5,67–69]. The cyclin B1 suppression prob- induced downregulation of Cdc25C protein levels.
ably prevents the optimal formation of the mitosis- Each of these mechanisms results in a decrease in or maturation-promoting factor (MPF) which con- Cdc2 kinase activity. Of particular interest is the sists of a complex between the cyclin B1 and the finding that the LOR-induced activation of Chk1 is Cdc2 kinase protein, and is essential for cells to cross implicated in each of these mechanisms of Cdc2 the threshold from G2 into mitosis. In this study, our regulation. Thus, studies in this report indicate that results revealed that cyclin B1 inhibition and its activation of Chk1 plays a central role, either directly inactivation of the cyclinB1/Cdc2 kinase activity or indirectly, in the induction of G2/M cell-cycle may be play an essential role in LOR-induced G2/M arrest and apoptosis by LOR through alterations in arrest. Such hypothesis has also been proposed in Cdc25C and BAD protein localization and activity.
previous studies revealed that cyclin B1 expression However, our study still needed to address a funda- in human cancer cells was inhibited by different mental question, which remains unanswered, is how stimulations including ionizing radiation [70], DNA LOR treatment causes DNA damage to activate ATR/ damaging agents [67], or cancer chemopreventive Chk1. Additional studies needed to identify the agents [5,71,72]. In addition, recent studies have specific mechanisms of LOR-mediated activation of shown that ectopic expression of BRCA1 in human Chk1, as well as the precise role of Chk1 on the cells can trigger cellular responses including G2/M regulation of G2/M arrest and apoptosis.
cell cycle arrest and apoptosis [68,69]. The BRCA1regulates the G2/M checkpoint was also demon- strated by activating Chk1 kinase and inhibition ofcyclin B1 upon DNA damage [69]. Similarly, our This study was supported by the National Science findings of decreased cyclin B1 expression concomi- Council grant NSC 92-2314-B-038-029 to Dr. Ho, and tant with the induction of Chk1 in the LOR-induced NSC 92-2320-B-038-018 to Dr. Lee.
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This Accepted Manuscript has not been copyedited and formatted. The final version may differ from this version. Research Article: New Research Sensory and Motor Systems Effects of fluoxetine and visual experience on glutamatergic andGABAergic synaptic proteins in adult rat visual cortex Effects of fluoxetine in adult rat V1 Simon Beshara , Brett R Beston