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Research Article: New Research Sensory and Motor Systems
Effects of fluoxetine and visual experience on glutamatergic and
GABAergic synaptic proteins in adult rat visual cortex
Effects of fluoxetine in adult rat V1 Simon Beshara , Brett R Beston
, Joshua GA Pinto
and Kathryn M Murphy
1McMaster Integrative Neuroscience Discovery and Study (MiNDS) Program, McMaster University2Department of Psychology, Neuroscience & Behavior, McMaster University3Department of Psychology, University of Toronto4Health Care Investment Banking, Credit Suisse AG Received: 14 October 2015 Revised: 4 December 2015 Accepted: 8 December 2015 Published: 24 December 2015 Author Contributions: SB Analyzed data, wrote the paper; BB designed research, performed research,
analyzed data; JP designed research, performed research, analyzed data; KM designed research, analyzed
data, wrote the paper.
Funding: Gouvernement du Canada Natural Sciences and Engineering Research Council of Canada (Conseil
de Recherches en Sciences Naturelles et en Génie du Canada): 501100000038; RGPIN-2015-06215.
Conflict of Interest: Authors report no conflict of interest
NSERC Grant RGPIN-2015-06215 awarded to KM.
Corresponding Author: Kathryn M Murphy, McMaster Integrative Neuroscience Discovery and Study(MiNDS) Program, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada. e-mail:[email protected] Cite as: eNeuro 2015; 10.1523/ENEURO.0126-15.2015
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Copyright 2015 Society for Neuroscience

Effects of fluoxetine and visual experience on glutamatergic and GABAergic synaptic proteins in adult rat visual cortex This is a confidential document and must not be discussed with others, forwarded in any form, or posted on websites without the express written consent of eNeuro.

1. Manuscript Title: Effects of fluoxetine and visual experience on glutamatergic and
GABAergic synaptic proteins in adult rat visual cortex 2. Abbreviated Title: Effects of fluoxetine in adult rat V1
3. Authors: Simon Beshara1, Brett R Beston1,2,a, Joshua GA Pinto1,b, Kathryn M
1, McMaster Integrative Neuroscience Discovery and Study (MiNDS) Program, McMaster University 2, Department of Psychology, Neuroscience & Behavior, McMaster University a, Department of Psychology, University of Toronto b, Health Care Investment Banking, Credit Suisse AG 4. Author Contributions:
SB Analyzed data, wrote the paper; BB designed research, performed research, analyzed data; JP designed research, performed research, analyzed data; KM designed research, analyzed data, wrote the paper. 5. Corresponding Author:
Kathryn M Murphy, McMaster Integrative Neuroscience Discovery and Study (MiNDS) Program, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada e-mail: [email protected] 6. Number of Figures: 5
7. Number of Tables: 1
8. Number of multimedia: 0
9. Number of Words for Abstract: 205
10. Number of words for Significance Statement: 121
11. Number of words for Introduction: 574
12. Number of words for Discussion: 1948
13. Acknowledgements: None
14. Conflict of interest: Authors report no conflict of interest
15. Funding Sources: NSERC Grant RGPIN-2015-06215 awarded to KM

Fluoxetine has emerged as a novel treatment for persistent amblyopia because in adult animals it reinstates critical period-like ocular dominance plasticity and promotes recovery of visual acuity. Translation of these results from animal models to the clinic, however, has been challenging because of the lack of understanding of how this SSRI affects glutamatergic and GABAergic synaptic mechanisms that are essential for experience-dependent plasticity. An appealing hypothesis is that fluoxetine recreates a CP-like state by shifting synaptic mechanisms to be more juvenile. To test this we studied the effect of fluoxetine treatment in adult rats, alone or in combination with visual deprivation (monocular deprivation, MD), on a set of highly conserved pre- and post-synaptic proteins (Synapsin, Synaptophysin, VGLUT1, VGAT, PSD-95, Gephyrin, GluN1, GluA2, GluN2B, GluN2A, GABAAα1, GABAAα3). We did not find evidence that fluoxetine shifted the protein amounts or balances to a CP-like state. Instead, it drove the balances in favor of the more mature subunits (GluN2A, GABAAα1). In addition, when fluoxetine was paired with MD it created a neuroprotective-like environment by normalizing the glutamatergic gain found in adult MDs. Together our results suggest that fluoxetine treatment creates a novel synaptic environment dominated by GluN2A- and GABAAα1-dependent plasticity.

Patching therapy is the most common treatment for children with amblyopia. For many, the acuity recovered during patching is lost when the treatment stops leaving the child with persistent amblyopia. Fluoxetine has emerged as an interesting treatment option because it reinstates critical period-like ocular dominance plasticity and promotes acuity recovery in adult animals. It remains unclear, however, how this selective serotonin reuptake inhibitor affects visual cortex plasticity, which relies heavily on glutamatergic and GABAergic synapses. Here we report the effects of fluoxetine and visual manipulation on the visual cortex of adult rats. Surprisingly we found that fluoxetine did not reinstate a critical period-like state, but rather created a novel synaptic environment that favors mature NMDA and GABAA receptor subunits.

Amblyopia is commonly treated with patching but in some cases the recovered acuity is lost when patching stops, leaving the child with persistent amblyopia (Birch, 2013). A variety of therapeutics have been proposed to treat persistent amblyopia in adolescents or young adults. Fluoxetine has emerged as a treatment option because it reinstates critical period-like (CP) ocular dominance plasticity and promotes acuity recovery in adult rats (Vetencourt et al., 2008). It is unclear, however, what effects this selective serotonin reuptake inhibitor (SSRI) has on visual cortex (V1) plasticity that relies heavily on maturation of glutamatergic and GABAergic synapses (Levelt and Hübener, 2012). An appealing idea is that fluoxetine shifts the synaptic environment in V1 to a CP-like state that supports heightened experience-dependent plasticity. During the CP, experience-dependent plasticity is driven by visually evoked responses that depend upon maturation of pre- and post-synaptic mechanisms. Development of presynaptic vesicle cycling proteins (e.g. Synapsin, Synaptophysin) and transporters (e.g. VGLUT, VGAT) are necessary for reliable neurotransmitter release (Hopf et al., 2002; Conti et al., 2004; Wojcik et al., 2004; 2006) that drives strong visually evoked activity. Also, shifts in the excitation:inhibition (E/I) balance set up the physiological environment needed for heightened plasticity, triggering the CP (Hensch, 2005; Hensch and Fagiolini, 2005). That E/I balance is mediate by postsynaptic scaffolding proteins PSD-95 and Gephyrin that regulate the number of excitatory and inhibitory synapses (Prange et al., 2004; Lardi-Studler et al., 2007; Keith and El-

Husseini, 2008). Furthermore, the start of the CP in rat and human V1 coincides with a rapid switch from much more Gephyrin to an equal balance with PSD-95 (Pinto et al., A host of glutamatergic and GABAergic receptor mechanisms affect the threshold for CP plasticity. These include addition of AMPA receptors (AMPAR) that end the period of NMDA receptor (NMDAR)-dominated silent synapses (Huang et al., 2015) and add the fast component to excitatory postsynaptic currents (EPSCs) (Kleppe and Robinson, 1999). Furthermore, the addition of GluN2A containing NMDARs (Flint et al., 1997; Stocca and Vicini, 1998) speeds up receptor kinetics (Cull-Candy et al., 2001) and affects signalling pathways such as GluN2B activation of Ras/ERK or alpha calcium-calmodulin kinase II and mTOR pathways (Kim et al., 2005; Wang et al., 2011). The shift to GluN2A also affects functional maturation by mediating sharpening of orientation selectivity (Fagiolini et al., 2003). Finally, activation of GABAA receptors (GABAAR) triggers the start of the CP (Hensch, 2005) and GABAAα1 subunits in particular regulate patterns of activity needed for development of ocular dominance (Fagiolini et al., 2004). Despite our understanding of the influence of fluoxetine treatment on adult plasticity, there is little evidence to identify 'how' fluoxetine affects the expression profile of synaptic mechanism that are critical in the initiation of CP plasticity. To address this, we treated animals with fluoxetine and quantified a set of glutamatergic and GABAergic synaptic proteins to assess if they changed to a CP-like state. We then determined the effects of monocular deprivation (MD) alone, or in combination with fluoxetine on these synaptic proteins. Surprisingly, fluoxetine alone shifted both NMDAR and GABAAR subunits to a more mature composition. Furthermore, when fluoxetine was combined with MD, the treatment normalized the increase in glutamatergic proteins found in adult MD rats. These results show that fluoxetine treatment does not recreate a CP-like synaptic environment but instead shifts plasticity mechanisms to a new state.
Materials and Methods Animals and surgical procedures
We studied changes in expression of 12 synaptic proteins in V1 of young adult male Long Evans rats (P98). Rats were individually housed in plexiglas cages with food and water ad libitum, and a 12:12 light/dark cycle. Animals were randomly assigned to 1 of 4 groups: normally reared to P98 (n=6), animals given 4 weeks of fluoxetine (from P70- P98) (0.2mg/ml of drinking water) (n=8), animals monocularly deprived (MDed) (P91- P98) (n=6), or animals that received both fluoxetine (P70-P98) and MD (P91-P98)(n=8). Eyelids were closed by trimming the lid margins and suturing them together with 5-0 vicryl using aseptic surgical techniques. The surgery was done using gaseous anesthetic [isoflurane (1.5–5%) in oxygen] for induction and maintenance of anesthesia. Eyelids were inspected daily for openings. All experimental procedures were approved by the [Authors' University] Animal Research Ethics Board. Tissue collection
Animals were euthanized (sodium pentobarbital, 150 mg/kg), and transcardially perfused with cold 0.1 M phosphate buffered saline (PBS) (4°C; 4-5 ml/min) until circulating fluid was clear. The brain was quickly removed from the skull and immersed in cold PBS. Bilateral samples of V1 including monocular and binocular regions, quickly frozen on dry ice, and stored at -80°C. Sample preparation
To study high-abundance vesicle cycling proteins and receptor scaffolding proteins (Synapsin, Synaptophysin, PSD-95, Gephyrin) we prepared homogenate samples. The frozen tissue was added to cold homogenization buffer (1 ml buffer:50 mg tissue – 0.5 mM DTT, 1mM EDTA, 2 mM EGTA, 10 mM HEPES, 10 mg/L leupeptin, 100 nM microcystin, 0.1 mM PMSF, 50 mg/L soybean trypsin inhibitor) and homogenized in a glass-glass Dounce homogenizer (Kontes, Vineland, NJ, USA). The sample was then combined with 10% sodium-dodecylsulfate (SDS). To study lower abundance receptor subunits (GluA2, GluN1, GluN2A, GluN2B, GABAAα1, GABAAα3) and transporters (VGLUT1, VGAT) we enriched the samples following a synaptoneurosomes protocol (Hollingsworth et al., 1985; Quinlan et al., 1999; Murphy et al., 2014). Following the homogenization step each sample was passed through a 5μm pore hydrophilic mesh filter (Millipore, Bedford, MA), then centrifuged at 1,000 g for 10 min. Both the synaptic pellet and the whole homogenate samples were resuspended in boiling 1% SDS. Protein concentrations for each sample was determined using the bicinchoninic acid (BCA) assay guidelines (Pierce, Rockford, IL, USA) and final concentrations were adjusted to 1mg/ml using Laemmli sample buffer. A control sample was made by combining a small amount of each of the 28 samples. Samples (25 μg) were resolved on 4-20% SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels (Precise Protein Gels, Pierce Biotechnology Inc., Rockford, IL, USA) and transferred to polyvinylidene difluoride (PVDF-FL) membranes (Millipore, Billerica, MA, USA). Blots were incubated with blocking buffer (Odyssey Blocking Buffer 1:1 with PBS) for 1 hour (LI-COR Biosciences; Lincoln, NE, USA), then with primary antibody overnight at 4°C using the following concentrations: GAPDH, 1:4000 (Imgenex, San Diego, CA); Synapsin 1, 1:8000 (Invitrogen, Carlsbad, CA); Synaptophysin, 1:2000 (Sigma-Aldrich, St. Louis, MO); PSD-95, 1:32000 (Millipore, Billerica, MA); Gephyrin, 1:2000 (Millipore, Billerica, MA); VGLUT1 , 1:2000 (Synaptic Systems, Goettingen, Germany); VGAT, 1:2000 (Synaptic Systems, Goettingen, Germany); GluA2, 1:2000 (Invitrogen, Carlsbad, CA); GluN1, 1:8000 (Chemicon International, Temecula, CA); GluN2B, 1:2000 (Chemicon International, Temecula, CA); GluN2A, 1:2000 (PhosphoSolutions, Aurora, CO); GABAAα1 1:500 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); GABAAα3 1:2000 (Chemicon International, Temecula, CA, USA). The blots were washed with PBS containing 0.05% Tween (Sigma, St. Louis, MO, USA) (PBS-T; 3 × 10 min), incubated for 1 hour at room temperature with the appropriate IRDye labeled secondary antibody, (Anti-Mouse, 1:8000, Anti-Rabbit, 1:10,000; LI-COR Biosciences; Lincoln, NE, USA), and washed in PBS-T (3 × 10 min). The blots were visualized using an Odyssey scanner (LI-COR Biosciences; Lincoln, NE, USA). The combination of IRDye secondary antibodies and Odyssey scanner provides a wide linear dynamic range so that both strong and weak bands could be accurately quantified on the same blot. Blots were stripped and re-probed with additional antibodies (Blot Restore Membrane Rejuvenation kit, Millipore, Billerica, MA, USA). Analyses
To analyze the bands, we scanned the blots (Odyssey infrared scanner) and quantified the bands using densitometry (LI-COR Odyssey Software version 3.0; LI- COR Biosciences; Lincoln, NE, USA). Density profiles were determined by performing a subtraction of the background, integrating the pixel intensity across the area of the band, and dividing the intensity by the width of the band to control for variations in lane width. Protein loading was checked using GAPDH as a control for sample concentration and volume loaded in each well. Each band was normalized to the average for the set of blots run at the same time and the control sample on the individual blot. To quantify the relationship between functional pairs of proteins we calculated contrast indices that are commonly used in signal processing to determine the quality of the signal. AMPAR-NMDAR Index -- (GluA2-GluN1)/(GluA2+GluN1). NMDAR Index -- (GluN2A-GluN2B)/(GluN2B+GluN2A). GABAAR Index -- (GABAA α1-GABAA α3)/(GABAA α1+GABAA α3). Presynaptic E/I Index -- (VGLUT1- VGAT)/(VGLUT1+VGAT). Postsynaptic E/I Index -- (PSD-95-Gephyrin)/(PSD- To compare levels of protein expression among the groups we made histograms showing the mean and standard error of the mean for each group. All results were plotted normalized to the normal young adult group. To make statistical comparisons between groups we used bootstrapping, a modern resampling statistical method that provides robust estimates of standard error and confidence intervals, that is especially useful for animal studies such as ours constrained to smaller sample sizes. We used R to simulate a normally distributed data set with 1,00,000 points and the same mean and standard deviation as the group being compared. To determine differences between groups we compared the simulation data set with average protein expression with each of the other groups. We ran a Monte Carlo simulation which randomly samples from the simulation data set N time, where N was the number of animals in each of the other groups (N=6 or 8). This simulation was repeated 10,000 times to create the normal distribution expected for the N sample sizes. We calculated confidence intervals for the simulated distribution and compared those with the observed means for the other groups. Groups were identified as significantly different (e.g. p < 0.05) when the observed average expression was either greater or less than 95% of the simulated distribution and thus outside its confidence interval. Image Manipulation
Bands are representative samples taken from different parts of the same gel or different gels. Horizontal and vertical transformations were uniformly applied to size bands appropriately for each figure. A linear adjustment layer was applied uniformly to all bands of each protein, preserving the relative intensities between groups.
We verified that GAPDH was an appropriate loading control by comparing expression of it among the 4 groups. We found no significant differences from normals demonstrating that GAPDH is an appropriate loading control. We began by examining expression of Synapsin, Snaptophysin, PSD-95 and Gephyrin in V1 ipsilateral to the deprived eye. MD effects are much weaker in the ipsilateral hemisphere (Sawtell et al., 2003) and we did not find any significant differences among the groups for those synaptic proteins (Fig. 1). Thus, all of the following analyses are for V1 contralateral to the deprived eye. Presynaptic changes
We analyzed how fluoxetine changed the presynaptic environment by quantifying a set of proteins involved in cycling, transport and loading of glutamatergic and GABAergic vesicles. We compared expression of Synapsin, Synaptophysin, VGLUT1 and VGAT in V1 of normally reared adults rats, rats given 1 month of fluoxetine, 1 week of MD, or the combination of fluoxetine and MD. We found no differences among the groups for Synapsin (n.s.) (Fig. 2A) or the GABAergic transporter VGAT (n.s.) (Fig. 2D) and only a modest loss of Synaptophysin for the MDed animals (-13% SEM 4.1%, p < 0.05) (Fig. 2B). The glutamate transporter VGLUT1 , however, had more changes. MDed animals had an increase in VGLUT1 (+25% SEM 8.4%, p < 0.001) while both groups of fluoxetine treated animals had less VGLUT1 than normal (fluoxetine alone -29% SEM 3.0%, p < 0.0001; fluoxetine+MD -13% SEM 4.9%, p < 0.05) (Fig. 2C). Postsynaptic changes
Next we examined how fluoxetine changed the expression of a set of postsynaptic scaffolding proteins and receptor subunits for glutamatergic and GABAergic receptors. Changes among the groups were very similar for PSD-95 and Gephyrin. Fluoxetine alone did not change the level of expression relative to normal animals, but MD caused loss of expression (PSD-95 -37% SEM 5.6%, p 0.06; Gephyrin -45% SEM 4.0%, p < 0.01) and fluoxetine combined with MD increased expression (PSD-95 +46% SEM 15%, p < 0.05; Gephyrin +34% SEM 11%, p < 0.05) (Fig. 3A-B). The pattern of changes for the receptor subunits was almost opposite to the scaffolding proteins. For the glutamatergic receptor subunits (GluN1, GluA2, GluN2B, GluN2A) fluoxetine alone caused losses for GluN1 and GluN2B (GluN1 -15% SEM 4.8%, p < 0.01; GluN2B -28% SEM 4.5 %, p < 0.01) and when combined with MD caused a loss of GluA2, as well as losses for GluN1 and GluN2B (GluA2 -15% SEM 4.2%, p < 0.0001; GluN1 -18% SEM 4.5%, p < 0.0001; GluN2B -21% SEM 4 %, p < 0.05) (Fig. 3C-F). But MD alone caused either an increase (GluN1 +25% SEM 8.8%, p < 0.0001; GluA2 +18% SEM 5.9%, p < 0.05) or no significant change from normal (GluN2B, GluN2A, n.s.). Thus, MD alone caused gains for these glutamatergic subunits that were reduced when MD was combined with fluoxetine. MD also increased GABAAα3 (+18% SEM 6.6%, p < 0.001) (Fig. 3G) but did not change GABAAα1 (Fig. 3H). In contrast, GABAAα1 was increased in both fluoxetine treated groups (fluoxetine alone +24% SEM 11%, p < 0.001; fluoxetine+MD +24% SEM 20%, p < 0.001) (Fig. 3H). Receptor subunit balances
During development there are a series of maturational shifts in expression of glutamatergic and GABAergic receptor subunits. One of the shifts is the change from NMDAR-dominated silent synapses to AMPAR activated synapses. We studied if fluoxetine created a CP-like state by shifting the GluA2:GluN1 balance in favour of GluN1 but found no changes from the normal adult balance (n.s.) (Fig. 4A). Different results were found when the GluN2A:GluN2B and GABAAα1:GABAAα3 balances were examined. During normal development there is an increase in GluN2A, shifting the balance from much more GluN2B to slightly in favour of GluN2B in young adult rats (Fig. 4B). But all of the experimental groups changed beyond that level towards even more GluN2A (p < 0.05). There were differences, however, in what drove the changes in the GluN2A:GluN2B balance with the fluoxetine groups shift being caused by less GluN2B while the MD shift was caused by more GluN2A. The GABAAα1:GABAAα3 balance revealed another dissociation among the experimental groups (Fig. 4C). Here the MD shift was caused by a 20% increase in GABAAα3 (p < 0.05), while the shift for the fluoxetine groups was caused by a 20% increase in GABAAα1 (fluoxetine alone, p < 0.01; fluoxetine+MD, p < 0.05) (Fig. 4C). This series of subunit balances unpacks subtle effect of fluoxetine treatment showing that it does not cause a shift to a CP-like state, instead it maintains subunit balances that are like normal adults (GluA2:GluN1) or shifted to more of the mature subunits (GluN2A, GABAAα1). E/I balances
The final analyses examined pre- and post-synaptic proteins that regulate the E/I balance. First, we calculated a presynaptic E/I balance using the vesicular transporters VGLUT1 and VGAT. MD caused a large shift towards VGLUT1 (p < 0.05) (Fig. 5A) but when combined with fluoxetine there was no change in the presynaptic E/I balance. The same pattern was seen on the postsynaptic side, here MD also caused a large shift towards the excitatory side (more PSD-95) (p < 0.05) (Fig. 5B) but when MD was paired with fluoxetine there was no change from the normal adult E/I balance.
In this study, we quantified the effect of fluoxetine treatment on 12 glutamatergic and GABAergic markers linked with visual experience-dependent plasticity in V1. Fluoxetine caused a pattern of change in those markers that provides new insights into how this drug affects plasticity in adult V1. We compared normal adult rats with ones treated with either fluoxetine alone, MD, or fluoxetine paired with MD. The main findings are that fluoxetine treatment in adult rats does not shift these markers to a younger pattern but instead rebalances MD driven glutamatergic gain and promotes a novel synaptic environment. In this study, we used Western blotting to quantify the effects of fluoxetine treatment on a collection of synaptic proteins in adult V1. A strength of this approach is that a large number of synaptic proteins were analyzed. Western blotting, however, does not provide laminar or cell specific information that is needed to identify the neural circuits in V1 affected by fluoxetine. Future neuroanatomical studies are needed to address that question and those studies may be guided by the current results. Fluoxetine does not recreate a younger synaptic environment
An appealing hypothesis about drug treatments, such as fluoxetine, is that they re- instate ocular dominance plasticity in adult V1 by changing the synaptic environment to a CP-like state. During the CP there are increases in amount of proteins and shifts in balances between functional pairs of synaptic proteins. Our results do not support the idea that fluoxetine in adult rats dials back synaptic age. For example, we found that fluoxetine combined with MD caused greater expression of PSD-95 and Gephyrin. These levels were higher than found during the CP (Pinto et al., 2015) and are consistent with a spike in PSD-95 that ends the CP (Huang et al., 2015). Furthermore, fluoxetine alone did not reduce expression of either scaffolding protein and only MD caused a loss of PSD-95 and Gephyrin. The modest losses for VGLUT1 and receptor subunits caused by fluoxetine suggest a shift to a more immature stage, but the balances among the subunits do not support that conclusion. If a younger synaptic environment was recreated then it should favour NMDA over AMPA receptors (Wu et al., 1996), GluN2B over GluN2A (Carmignoto and Vicini, 1992; Flint et al., 1997; Stocca and Vicini, 1998) and GABAAα3 over GABAAα1 (Laurie et al., 1992). Instead, the NMDAR to AMPAR ratios were balanced for both fluoxetine groups (GluN1 GluA2), while subunit balances jumped past age matched adults towards even more of the mature subunits (GluN2A, GABAAα1). Finally, E/I balances for pre- and post-synaptic markers were similar to the normal adults in both fluoxetine groups. Together these findings illustrate that fluoxetine treatment did not simply recreate a CP-like synaptic environment in V1. It is important to note that we examined synaptic proteins after 1 month of fluoxetine treatment and 1wk of MD. We know from previous studies (Williams et al., 2015) that there are dynamic changes in synaptic proteins during a period of MD and it seems reasonable to propose that fluoxetine treatment may cause similarly dynamic changes. Thus the findings here provide a snapshot of long-term effects of fluoxetine treatment. It will be important for future studies to probe other time points to understand the full landscape of synaptic changes and how transient changes caused by fluoxetine (Vetencourt et al., 2011) impact long-term plasticity in V1. Fluoxetine triggers a novel synaptic environment in adult V1
The original study showing that fluoxetine reinstates ocular dominance plasticity also found improvement of visual function, reduced intracortical inhibition, and increased BDNF expression (Vetencourt et al., 2008). All of those changes occurred without significantly altering neuronal responsiveness or orientation selectivity in V1. Here we found normal pre- and post-synaptic E/I balances, and adult levels of GABAAα1 that could support normal responsiveness and orientation selectivity. A previous study of gene expression found reduced VGAT after fluoxetine treatment but no changes in other genes associated with E/I mechanisms (Tiraboschi et al., 2013). We, however, did not find that fluoxetine caused a loss of VGAT protein expression. Some forms of GABAergic plasticity involve changes in VGAT protein expression associated with the amount of neurotransmitter in vesicles (Hartman et al., 2006), and the lack of change in VGAT makes it unlikely that fluoxetine altered this type of plasticity. A recent proteomic analysis found that fluoxetine caused alterations in cytoskeleton organization, endocytosis, molecular transport, intracellular signaling, redox cellular state, metabolism and protein degradation (Ruiz-Perera et al., 2015). Those changes included proteins that regulate AMPAR and GABAAR, and may affect the E/I balance. Nonetheless, our quantification of synaptic proteins, along with the gene and proteomic studies, show that fluoxetine affects mechanisms that regulate experience-dependent The GluN2A:GluN2B and GABAAα1:GABAAα3 balances were both affected by fluoxetine and importantly the GABAA balance differentiated fluoxetine treatments from the effects of MD. The changes in these functional pairs of glutamatergic and GABAergic receptor subunits suggest that fluoxetine creates a novel synaptic environment in adult V1. An environment that is dominated by GluN2A and GABAAα1 but also has balanced levels of pre- and post-synaptic E/I markers. Both GluN2A and GABAAα1 subunits are described as mature components because they gradually increase during development and affect plasticity. For example, the developmental shift from GluN2B to more GluN2A speeds up receptor kinetics (Cull- Candy et al., 2001), changes cellular signalling (Kim et al., 2005; Wang et al., 2011), relieves GluN2B negative regulation of AMPARs (Hall and Ghosh, 2008), and controls metaplasticity in V1 (Philpot et al., 2007). GABAAα1 is necessary for normal development of orientation tuning (Fagiolini et al., 2004) and gamma rhythms (Cardin et al., 2009; Sohal et al., 2009). The prevalence of GABAAα1 positive synapses on pyramidal cell bodies makes them important components in GABAergic regulation of experience-dependent plasticity (Hensch, 2005; Griffen and Maffei, 2014). The different roles of these subunits in experience-dependent plasticity suggests that fluoxetine creates a unique synaptic environment in adult V1 that can support both GluN2A- dependent metaplasticity and GABAergic regulation of ocular dominance plasticity. How might fluoxetine trigger adult plasticity?
Reduced intracortical GABA and GABAergic transmission have been found after fluoxetine treatment (Vetencourt et al., 2008; Baroncelli et al., 2011). In contrast, we found a small increase in GABAAα1 expression and no loss of GABAAα3 or VGAT in rats treated with fluoxetine. Previous studies have shown that fluoxetine positively modulates GABAA receptors and one way is by increasing receptor sensitivity to small amounts of GABA (Robinson, 2002). The α1 subunit is one of the subtypes that confers that increased sensitivity and perhaps more GABAAα1 expression modulates GABAergic drive when the amount of neurotransmitter is reduced by fluoxetine. Interestingly, during the CP a brief exposure to vision after deprivation causes a rapid rebound potentiation in mini inhibitory postsynaptic currents (mIPSCs) that is correlated with an increase in GABAARs (Gao et al., 2014). Perhaps the increase in GABAAα1 expression found here supports a similar potentiation of mIPSCs and since GABAAα1 containing synapses form a key part of the neural circuitry involved in ocular dominance plasticity (Hensch, 2005) fluoxetine may drive a compensatory mechanisms where sensitized GABAARs enhance adult plasticity. We also found that fluoxetine caused changes to glutamatergic receptor subunit expression. Fluoxetine is known to inhibit NMDA receptors and may provide neuroprotective effects by regulating glutamatergic involvement in excitotoxicity (Szasz et al., 2007). We found that fluoxetine paired with MD ameliorated glutamatergic gain driven by MD alone, suggesting that one of fluoxetine's effects in adult V1 may be neuroprotective. Fluoxetine acts by inhibiting GluN2B containing NMDARs (Kiss et al., 2012) and that may trigger increases in both BDNF and AMPARs. GluN2B-mediated signalling inhibits AMPAR trafficking and the amount of GluA2 containing AMPARs (Kim et al., 2005; Derkach et al., 2007; Hall et al., 2007; Hall and Ghosh, 2008) through unique cellular processes that include Ras/ERK, αCamKII, and mTor pathways (Kim et al., 2005; Wang et al., 2011). One way that fluoxetine could affect adult ocular dominance plasticity is if the loss of GluN2B changes the length of GluN2B-mediated Ras/ERK activation (Kim et al., 2005) thereby increasing insertion of AMPAR into synapses and supporting long-term potentiation (LTP). ERK activation is necessary for ocular dominance plasticity in developing V1 (Di Cristo et al., 2001) and fluoxetine in adult animals may enhance ERK-dependent plasticity through the loss of GluN2B. During the CP ocular dominance plasticity reflects the depression of deprived eye responses but in adults MD leads to enhancement of open eye responses in V1 (Sawtell et al., 2003). That adult plasticity is dependent on activation of NMDARs and may use hebbian (LTP, long-term depression LTD, spike time dependent plasticity), homeostatic, or metaplasticity (synaptic modification threshold) mechanisms (for review, see Hofer et al., 2006). Visual experience driven changes to LTP and LTD during the CP depend on GluN2A and previous studies have identified shifts in the GluN2A:GluN2B balance as the mechanism underlying an adjustable synaptic modification threshold in V1 (Philpot et al., 2007). Perhaps the shift to balanced GluN2A:GluN2B expression after fluoxetine treatment is an indication that metaplasticity plays a dominant role in fluoxetine driven adult plasticity. Interestingly, in auditory cortex fluoxetine reduces the potential for LTP (Dringenberg et al., 2014) raising the possibility that the effects of fluoxetine might not be uniform across the cortex. Fluoxetine could also trigger events similar to those promoted by other NMDAR antagonists that cause a transient burst of glutamate, followed by BDNF release and synapse formation (Duman and Aghajanian, 2014). BDNF plays a key role in fluoxetine's reactivation of plasticity (Castrén and Rantamäki, 2010) suggesting that a fluoxetine induced loss of GluN2B signalling may enhance BDNF and AMPAR involvement in experience-dependent plasticity in adult V1. Thus, fluoxetine appears to enhance glutamatergic and GABAergic mechanisms that support experience- dependent plasticity in adult V1. Implications for other therapies
A variety of other methods are being explored to promote adult recovery from persistent amblyopia, such as dark rearing in animals (He et al., 2006; 2007; Montey and Quinlan, 2011; Duffy and Mitchell, 2013), manipulation of the brakes on plasticity including PirB (Bochner et al., 2014) and chondroitin sulphate proteoglycans (CSPGs) (Pizzorusso et al., 2002; Morishita et al., 2010; Bukhari et al., 2015), environmental enrichment (Sale et al., 2007), patterned visual stimulation (Montey et al., 2013), or perceptual learning (Levi and Li, 2009; Baroncelli et al., 2011; Bonaccorsi et al., 2014; Tsirlin et al., 2015). All of these appear to reactivate a certain degree of plasticity that can support ocular dominance plasticity and even visual recovery. The cellular mechanisms typically include LTP of cortical synapses, and although some molecular changes have been identified (He et al., 2006), the full extent has yet to be explored. Do these other techniques mimic the novel pattern of fluoxetine driven glutamatergic and GABAergic changes or do they create different synaptic environments? These are important questions to answer to determine if these adult manipulations activate one or many different forms of experience-dependent plasticity in V1. Future studies will need to determine the long-term consequences of fluoxetine- induced changes in adult V1. It is not clear if stopping drug treatment will allow the synaptic environment to shift back to a normal adult state. And if not what effects that new synaptic environment may have on neural function in the long-term. Finally, it will be important to determine how much of these effects are driven by the increase in serotonin, as opposed to unique effects of fluoxetine. Each of these are important questions to answer that well help to understand plasticity in adult V1 and translate that knowledge into effective treatments for persistent amblyopia. 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Fig 1. Pre- and post-synaptic proteins in ipsilateral V1. In V1 ipsilateral to the
deprived eye, there was no effect of experimental condition on the expression of Synapsin (A), Synaptophysin (B), PSD-95 (C), or Gephyrin (D) (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Fig 2. Presynaptic vesicle cycling and transporter proteins. In contralateral V1,
Synapsin (A) was not affected by experimental condition. For Synaptophysin (B) fluoxetine alone had no effect, MD alone caused a loss of expression, but combining fluoxetine with MD prevented the MD-induced loss. For VGLUT1 (C) fluoxetine alone or with MD caused a loss of expression, but MD alone increased expression. VGAT (D) was not affected by experimental condition. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < Fig 3. Postsynaptic receptor scaffolding proteins and subunits. In contralateral V1,
PSD-95 (A) and Gephyrin (B) had a similar pattern of changes: fluoxetine alone had no effect, MD alone caused a loss of expression, but combining fluoxetine with MD prevented the MD-induced loss and caused super-compensation above normal levels. GluN1 (C) was reduced by fluoxetine regardless of visual experience, while MD alone caused an increase. GluA2 (D) was unaffected by fluoxetine alone, MD caused an increase, but combing fluoxetine with MD caused a decrease. GluN2B (E) was reduced by fluoxetine regardless of visual experience, while MD had no effect. GluN2A (F) expression of each experimental group was not different from normal animals, but the MDed group had higher expression than either fluoxetine alone or fluoxetine combined with MD. GABAAα3 (G) was unaffected by fluoxetine alone, MD caused an increase, but combing fluoxetine with MD prevented the MD-induced increase. GABAAα1 (H) was increased by fluoxetine regardless of visual experience, while MD alone had no effect. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Fig 4. Postsynaptic receptor subunit balances. Neither fluoxetine, MD, nor fluoxetine
combined with MD affected the relative abundance of GluN1-containing NMDARs and GluA2-containing AMPARs in contralateral V1 (A). Fluoxetine shifted the relative abundance of NMDAR subunits in favour of the more mature GluN2A subunit, regardless of visual experience. MD caused a shift in favour of the more immature GluN2B (B). Fluoxetine shifted the relative abundance of GABAAR subunits in favour of the more mature α1 subunit, regardless of visual experience. MD caused a shift in favour of the more α3 subunit (C). (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Fig 5. Pre- and Post-synaptic E/I balance. Presynaptic Index in contralateral V1 (A):
(VGLUT1-VGAT)/(VGLUT1+VGAT). Postsynaptic Index in contralateral V1 (B): (PSD- 95-Gephyrin)/(PSD-95+Gephyrin). We found strikingly similar patters in the pre- and post-synaptic indexes of E/I synapses. Fluoxetine caused a slight shift towards inhibition in the presynaptic index and had no effect on the postsynaptic index. MD caused a strong shift to excitatory markers. Combining fluoxetine and MD kept the balance at normal levels. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Table 1. Statistical table. Statistical comparisons were made by using Bootstrapping
and Monte Carlo Simulations to generate expected 95% confidence intervals for each group being tested.
interval vs Normal interval vs 1wk MD Fluoxetine + 1wk V1 Ipsi Synapsin - Bootstrapping + 0.8112-1.1888 Monte Carlo Simulation V1 Ipsi Synapsin- Bootstrapping + 0.8388-1.1612 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8094-1.1906 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8378-1.1622 Fluoxetine + 1wk MD Monte Carlo Simulation Bootstrapping + 0.8812-1.1188 Bootstrapping + 0.8957-1.1043 Bootstrapping + 0.8783-1.1217 Synaptophysin - 1wk Bootstrapping + 0.8977-1.1023 Fluoxetine + 1wk MD V1 Ipsi PSD-95 - Bootstrapping + 0.6143-1.3857 Monte Carlo Simulation V1 Ipsi PSD-95 - Bootstrapping + 0.6678-1.3322 Monte Carlo Simulation V1 Ipsi PSD-95 - 1wk Normal Bootstrapping + 0.6117-1.3883 Monte Carlo Simulation V1 Ipsi PSD-95 - Bootstrapping + 0.6678-1.3322 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Ipsi Gephyrin - Bootstrapping + 0.7124-1.2876 Monte Carlo Simulation V1 Ipsi Gephyrin - Bootstrapping + 0.7491-1.2509 Monte Carlo Simulation V1 Ipsi Gephyrin - Bootstrapping + 0.7048-1.2952 Monte Carlo Simulation V1 Ipsi Gephyrin - Bootstrapping + 0.7533-1.2467 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra Synapsin- Normal Bootstrapping + 0.8105-1.1895 Monte Carlo Simulation interval vs Normal interval vs 1wk MD Fluoxetine + 1wk V1 Ipsi Synapsin - Bootstrapping + 0.8112-1.1888 Monte Carlo Simulation V1 Ipsi Synapsin- Bootstrapping + 0.8388-1.1612 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8094-1.1906 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8378-1.1622 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra Synapsin- Normal Bootstrapping + 0.8384-1.1616 Monte Carlo Simulation V1 Contra Synapsin - Normal Bootstrapping + 0.8138-1.1862 Monte Carlo Simulation V1 Contra Synapsin - Normal Bootstrapping + 0.8381-1.1619 Fluoxetine + 1wk MD Monte Carlo Simulation Bootstrapping + 0.8801-1.1199 Bootstrapping + 0.8777-1.1223 Bootstrapping + 0.8959-1.1041 Synaptophysin - 1wk Bootstrapping + 0.8930-1.1070 Fluoxetine + 1wk MD V1 Contra VGLUT1 - Normal Bootstrapping + 0.8693-1.1307 Monte Carlo Simulation V1 Contra VGLUT1 - Normal Bootstrapping + 0.8872-1.1128 Monte Carlo Simulation V1 Contra VGLUT1 - Normal Bootstrapping + 0.8685-1.1315 Monte Carlo Simulation V1 Contra VGLUT1 - Normal Bootstrapping + 0.8876-1.1124 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra VGAT - Bootstrapping + 0.6458-1.3542 Monte Carlo Simulation V1 Contra VGAT - Bootstrapping + 0.6993-1.3007 Monte Carlo Simulation V1 Contra VGAT - Bootstrapping + 0.6512-1.3488 Monte Carlo Simulation interval vs Normal interval vs 1wk MD Fluoxetine + 1wk V1 Ipsi Synapsin - Bootstrapping + 0.8112-1.1888 Monte Carlo Simulation V1 Ipsi Synapsin- Bootstrapping + 0.8388-1.1612 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8094-1.1906 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8378-1.1622 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra VGAT - Bootstrapping + 0.6956-1.3044 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra PSD-95 - Normal Bootstrapping + 0.6038-1.3962 Monte Carlo Simulation V1 Contra PSD-95 - Normal Bootstrapping + 0.6683-1.3317 Monte Carlo Simulation V1 Contra PSD-95 - Normal Bootstrapping + 0.6097-1.3903 Monte Carlo Simulation V1 Contra PSD-95 - Normal Bootstrapping + 0.6679-1.3321 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra Gephyrin - Normal Bootstrapping + 0.7050-1.2950 Monte Carlo Simulation V1 Contra Gephyrin - Normal Bootstrapping + 0.7480-1.2520 Monte Carlo Simulation V1 Contra Gephyrin - Normal Bootstrapping + 0.7089-1.2911 Monte Carlo Simulation V1 Contra Gephyrin - Normal Bootstrapping + 0.7515-1.2485 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra GluN1 - Bootstrapping + 0.8909-1.1092 Monte Carlo Simulation V1 Contra GluN1 - Bootstrapping + 0.9037-1.0963 Monte Carlo Simulation V1 Contra GluN1 - Bootstrapping + 0.8910-1.1090 Monte Carlo Simulation V1 Contra GluN1 - Bootstrapping + 0.9053-1.0947 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra GluA2 - Bootstrapping + 0.8632-1.1368 Monte Carlo Simulation interval vs Normal interval vs 1wk MD Fluoxetine + 1wk V1 Ipsi Synapsin - Bootstrapping + 0.8112-1.1888 Monte Carlo Simulation V1 Ipsi Synapsin- Bootstrapping + 0.8388-1.1612 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8094-1.1906 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8378-1.1622 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra GluA2 - Bootstrapping + 0.8824-1.1176 Monte Carlo Simulation V1 Contra GluA2 - Bootstrapping + 0.8605-1.1395 Monte Carlo Simulation V1 Contra GluA2 - Bootstrapping + 0.8790-1.1210 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra GluN2A - Normal Bootstrapping + 0.7104-1.2896 Monte Carlo Simulation V1 Contra GluN2A - Normal Bootstrapping + 0.7063-1.2937 Monte Carlo Simulation V1 Contra GluN2A - Normal Bootstrapping + 0.7430-1.2569 Monte Carlo Simulation V1 Contra GluN2A - Normal Bootstrapping + 0.7418-1.2582 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra GluN2B - Normal Bootstrapping + 0.7772-1.2228 Monte Carlo Simulation V1 Contra GluN2B - Normal Bootstrapping + 0.7812-1.2188 Monte Carlo Simulation V1 Contra GluN2B - Normal Bootstrapping + 0.8074-1.1926 Monte Carlo Simulation V1 Contra GluN2B - Normal Bootstrapping + 0.8120-1.1880 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra GABAA3 Normal Bootstrapping + 0.8712-1.1288 Monte Carlo Simulation V1 Contra GABAA3 Normal Bootstrapping + 0.8908-1.1092 Monte Carlo Simulation V1 Contra GABAA3 Normal Bootstrapping + 0.8729-1.1271 Monte Carlo Simulation interval vs Normal interval vs 1wk MD Fluoxetine + 1wk V1 Ipsi Synapsin - Bootstrapping + 0.8112-1.1888 Monte Carlo Simulation V1 Ipsi Synapsin- Bootstrapping + 0.8388-1.1612 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8094-1.1906 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8378-1.1622 Fluoxetine + 1wk MD Monte Carlo Simulation V1 Contra GABAA3 Normal Bootstrapping + 0.8894-1.1106 - Fluoxetine + 1wk V1 Contra GABAA1 Normal Bootstrapping + 0.8751-1.1249 Monte Carlo Simulation V1 Contra GABAA1 Normal Bootstrapping + 0.8898-1.1102 Monte Carlo Simulation V1 Contra GABAA1 Normal Bootstrapping + 0.8713-1.1287 Monte Carlo Simulation V1 Contra GABAA1 Normal Bootstrapping + 0.8883-1.1117 - Fluoxetine + 1wk Bootstrapping + -0.0675-0.0603 Bootstrapping + -0.0595-0.0523 Bootstrapping + -0.0675-0.0603 GluA2:GluN1 - 1wk Bootstrapping + -0.0596-0.0525 Fluoxetine + 1wk MD Bootstrapping + -0.1879-0.0107 Bootstrapping + -0.1755-(-0.0018) -0.0553-0.0735 Bootstrapping + -0.1862-0.0090 Bootstrapping + -0.1738-(-0.0034) -0.0569-0.0752 Fluoxetine + 1wk MD Bootstrapping + -0.1582-0.0079 -0.2900-(-0.0325) -0.1058-0.1577 interval vs Normal interval vs 1wk MD Fluoxetine + 1wk V1 Ipsi Synapsin - Bootstrapping + 0.8112-1.1888 Monte Carlo Simulation V1 Ipsi Synapsin- Bootstrapping + 0.8388-1.1612 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8094-1.1906 Monte Carlo Simulation V1 Ipsi Synapsin - Bootstrapping + 0.8378-1.1622 Fluoxetine + 1wk MD Monte Carlo Simulation Bootstrapping + -0.1463-(-0.0039) -0.0619-0.1650 -0.2744-(-0.0481) -0.0873-0.1392 Bootstrapping + -0.1594-0.0092 -0.2919-(-0.0306) -0.1062-0.1582 Bootstrapping + -0.1472-(-0.0031) -0.0610-0.1641 -0.2729-(-0.0496) -0.0866-0.1385 Fluoxetine + 1wk MD Bootstrapping + -0.0981-0.1807 Presynaptic E/I - Bootstrapping + -0.0809-0.1635 Presynaptic E/I - Bootstrapping + -0.0983-0.1808 Presynaptic E/I - 1wk Bootstrapping + -0.0770-0.1595 Presynaptic E/I - Fluoxetine + 1wk MD Bootstrapping + -0.1202-0.1499 Postsynaptic E/I - Bootstrapping + -0.0999-0.1295 Postsynaptic E/I - Bootstrapping + -0.1199-0.1495 Postsynaptic E/I - Bootstrapping + -0.1008-0.1304 Postsynaptic E/I - Fluoxetine + 1wk MD

Source: http://eneuro.org/content/eneuro/early/2015/12/24/ENEURO.0126-15.2015.full.pdf